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Titolo:
Synthesis of progesterone in Schwann cells: regulation by sensory neurons
Autore:
Robert, F; Guennoun, R; Desarnaud, F; Do-Thi, A; Benmessahel, Y; Baulieu, EE; Schumacher, M;
Indirizzi:
INSERM, U488, F-94276 Le Kremlin Bicetre, France INSERM Le Kremlin Bicetre France F-94276 4276 Le Kremlin Bicetre, France
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 5, volume: 13, anno: 2001,
pagine: 916 - 924
SICI:
0953-816X(200103)13:5<916:SOPISC>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERIPHERAL MYELIN PROTEIN-22; BINDING INHIBITOR RECEPTOR; STEROID-HORMONES; GENE-EXPRESSION; IN-VIVO; NEUROSTEROIDS; PREGNENOLONE; BRAIN; REGENERATION; METABOLISM;
Keywords:
3 beta-hydroxysteroid dehydrogenase; myelin sheaths; nerve regeneration; neurosteroids; rats;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Schumacher, M INSERM, U488, 80 Rue Gen Leclerc, F-94276 Le Kremlin Bicetre, France INSERM 80 Rue Gen Leclerc Le Kremlin Bicetre France F-94276
Citazione:
F. Robert et al., "Synthesis of progesterone in Schwann cells: regulation by sensory neurons", EUR J NEURO, 13(5), 2001, pp. 916-924

Abstract

In peripheral nerves, progesterone synthesized by Schwann cells has been implicated in myelination. In spite of such an important function, little isknown of the regulation of progesterone biosynthesis in the nervous system. We show here that in rat Schwann cells, expression of the 3 beta -hydroxysteroid dehydrogenase and formation of progesterone are dependent on neuronal signal. Levels of 3 beta -hydroxysteroid dehydrogenase mRNA and synthesis of [H-3]progesterone from [H-3]pregnenolone were low in purified Schwann cells prepared from neonatal rat sciatic nerves. However, when Schwann cells were cultured in contact with sensory neurons, both expression and activity of the 3 beta -hydroxysteroid dehydrogenase were induced. Regulation of 3 beta -hydroxysteroid dehydrogenase expression by neurons was also demonstrated in vivo in the rat sciatic nerve. 3 beta -hydroxysteroid dehydrogenase mRNA was present in the intact nerve, but could no longer be detected 3 or 6 days after cryolesion, when axons had degenerated. After 15 days, when Schwann cells made new contact with the regenerating axons, the enzyme was re-expressed. After nerve transection, which does not allow axonal regeneration, 3 beta -hydroxysteroid dehydrogenase mRNA remained undetectable. The regulation of 3 beta -hydroxysteroid dehydrogenase mRNA after lesion was similar to the regulation of myelin protein zero (P-0) and peripheral myelin protein 22 (PMP22) mRNAs, supporting an important role of locally formed progesterone in myelination.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 07:35:48