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Titolo:
The alpha-amino group of L-arginine mediates its antioxidant effect
Autore:
Wallner, S; Hermetter, A; Mayer, B; Wascher, TC;
Indirizzi:
Graz Univ, Dept Internal Med, A-8036 Graz, Austria Graz Univ Graz Austria A-8036 v, Dept Internal Med, A-8036 Graz, Austria Graz Tech Univ, Dept Biochem, Graz, Austria Graz Tech Univ Graz AustriaGraz Tech Univ, Dept Biochem, Graz, Austria
Titolo Testata:
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
fascicolo: 2, volume: 31, anno: 2001,
pagine: 98 - 102
SICI:
0014-2972(200102)31:2<98:TAGOLM>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIUM-DEPENDENT DILATION; VITAMIN-C; YOUNG MEN; DYSFUNCTION; RELAXATION; SUBSTRATE; OXIDATION; INJURY; CELLS;
Keywords:
antioxidants; atherosclerosis; L-arginine; lipoprotein oxidation; nitric oxide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Wascher, TC Graz Univ, Dept Internal Med, Auenbruggerpl 15, A-8036 Graz, Austria Graz Univ Auenbruggerpl 15 Graz Austria A-8036 Graz, Austria
Citazione:
S. Wallner et al., "The alpha-amino group of L-arginine mediates its antioxidant effect", EUR J CL IN, 31(2), 2001, pp. 98-102

Abstract

Background Antioxidant effects may constitute part of the possible antiatherogenic effects of the amino acid L-arginine. These antioxidant propertieswere further characterized in a model of lipoprotein oxidation. Methods and Results Oxidation of lipoproteins in unfractionated human serum was continuously monitored by a fluorescent probe. The antioxidant effects of L-arginine, N-alpha -acetyl-arginine and vitamin E in combination withL-arginine were measured after initiation of free radical generation with either copper or 2,2'-azobis(2-amidinopropane) hydrochloride (AAPH). The half-time of the fast propagation rate for copper-induced lipoprotein oxidation increased after incubation with L-arginine in a dose-dependent manner (P< 0.01). N-<alpha>-acetyl-arginine did not show such effects. Vitamin E and L-arginine show different effects on copper-induced oxidation, the formerincreasing only lag-time, the latter increasing only propagation rate, anddo not have reciprocal effects. In contrast to copper-induced oxidation, L-arginine increased the lag-time of AAPH-induced lipoprotein oxidation (P <0.01), with no effect on the propagation rate at physiological concentrations. Again, N-<alpha>-acetyl-arginine did not show any antioxidant effects. Conclusion Our experiments provide further evidence that mechanisms other than serving as a substrate for the NO-synthase could be involved in the antiatherosclerotic effect of L-arginine. In addition, our experiments clearly show, that the antioxidant effect of L-arginine is due to a chemical moiety different from that serving as the substrate for NO biosynthesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:19:43