Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Resistance to induced apoptosis in the human neuroblastoma cell line SK-N-SH in relation to neuronal differentiation - Role of Bcl-2 protein family
Autore:
Lombet, A; Zujovic, V; Kandouz, M; Billardon, C; Carvajal-Gonzalez, S; Gompel, A; Rostene, W;
Indirizzi:
Hop St Antoine, INSERM, U339, F-75012 Paris, France Hop St Antoine ParisFrance F-75012 INSERM, U339, F-75012 Paris, France
Titolo Testata:
EUROPEAN JOURNAL OF BIOCHEMISTRY
fascicolo: 5, volume: 268, anno: 2001,
pagine: 1352 - 1362
SICI:
0014-2956(200103)268:5<1352:RTIAIT>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
SH-SY5Y CELLS; KINASE-C; E1B 19K; IN-VIVO; EXPRESSION; DEATH; BCL-X(L); CHANNEL; MICE; BAX;
Keywords:
protein kinase C; Bax; Bak; Bcl-xL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Lombet, A Hop St Antoine, INSERM, U339, 184 Rue Faubourg, F-75012 Paris, France Hop St Antoine 184 Rue Faubourg Paris France F-75012 is, France
Citazione:
A. Lombet et al., "Resistance to induced apoptosis in the human neuroblastoma cell line SK-N-SH in relation to neuronal differentiation - Role of Bcl-2 protein family", EUR J BIOCH, 268(5), 2001, pp. 1352-1362

Abstract

Much evidence suggests that apoptosis plays a crucial role in cell population homeostasis that depends on the expression of various genes implicated in the control of cell life and death. The sensitivity of human neuroblastoma cells SK-N-SH to undergo apoptosis induced by thapsigargin was examined. SK-N-SH were previously differentiated into neuronal cells by treatments with retinoic acid (RA), 4 beta -phorbol 12-myristate 13-acetate (PMA) whichincreases protein kinase C (PKC) activity, and staurosporine which decreases PKC activity. Neuronal differentiation was evaluated by gamma -enolase, microtubule associated protein 2 (MAP2) and synaptophysin immunocytochemistry. The sensitivity of the cells to thapsigargin-induced apoptosis was evaluated by cell viability and nuclear fragmentation (Hoechst 33258) and compared with pro-(Bcl-2, Bcl-x(L)) and anti-apoptotic (Bax, Bak) protein expression of the Bcl-2 family. Cells treated with RA and PMA were more resistant to apoptosis than controls. Conversely, the cells treated with staurosporine were more susceptible to apoptosis. In parallel with morphological modifications, the expression of inhibitors and activators of apoptosis was directly dependent upon the differentiating agent used. Bcl-2 expression was strongly increased by PMA and drastically decreased by staurosporine as was Bcl-x(L) expression. Bax and Bak expression were not significantly modified. These results demonstrate that drugs that modulate PKC activity may induce a modification of Bcl-2 expression as well as resistance to the apoptotic process. Furthermore, theexpression of Bcl-2 was reduced by toxin B from Clostridium difficile and,to a lesser extent, by wortmannin suggesting a role of small G-protein RhoA and PtdIns3 kinase in the control of Bcl-2 expression. Our data demonstrate a relationship between the continuous activation of PKC, the expression of Bcl-2 protein family and the resistance of differentiated SK-N-SH to apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:42:08