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Titolo:
Up-regulation of mucin secretion in HT29-MTX cells by the pro-inflammatorycytokines tumor necrosis factor-alpha and interleukin-6
Autore:
Smirnova, MG; Kiselev, SL; Birchall, JP; Pearson, JP;
Indirizzi:
Univ Newcastle Upon Tyne, Sch Med, Dept Physiol Sci, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England Univ Newcastle Upon Tyne Newcastle Upon Tyne Tyne & Wear England NE2 4HH Russian Acad Sci, Inst Gene Biol, Dept Mol Genet Canc, Moscow 117334, Russia Russian Acad Sci Moscow Russia 117334 Genet Canc, Moscow 117334, Russia Univ Nottingham Hosp, Queens Med Ctr, Dept Otorhinolaryngol, Nottingham NG7 2UH, England Univ Nottingham Hosp Nottingham England NG7 2UH tingham NG7 2UH, England
Titolo Testata:
EUROPEAN CYTOKINE NETWORK
fascicolo: 1, volume: 12, anno: 2001,
pagine: 119 - 125
SICI:
1148-5493(200103)12:1<119:UOMSIH>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
MIDDLE-EAR EFFUSIONS; NF-KAPPA-B; OTITIS-MEDIA; GENE-EXPRESSION; IN-VITRO; MUC5B; IDENTIFICATION; PROTEINS; TNF; ORGANIZATION;
Keywords:
MUC5AC; MUC5B; mucus-secreting cell line HT29-MTX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Smirnova, MG Univ Newcastle Upon Tyne, Sch Med, Dept Physiol Sci, Framlington Pl, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England Univ Newcastle Upon Tyne Framlington Pl Newcastle Upon Tyne Tyne & Wear England NE2 4HH
Citazione:
M.G. Smirnova et al., "Up-regulation of mucin secretion in HT29-MTX cells by the pro-inflammatorycytokines tumor necrosis factor-alpha and interleukin-6", EUR CYTOKIN, 12(1), 2001, pp. 119-125

Abstract

The pro-inflammatory cytokines IL-6 and TNF-alpha have been implicated in the pathogenesis of otitis media with effusion (OME), A disease where goblet cells proliferate in a modified respiratory epithelium, leading to the accumulation of a mucin-rich effusion in the middle ear cleft. The MUC5AC andMUC5B mucin gene products have been identified as components of these effusions, To determine the effect of IL-6 and TNF-a on MUC5AC and MUC5B secretion we have used HT29-MTX goblet cells, which secrete both types of mucins,MUC5AC and MUC5B mucin secretion was measured by an enzyme-linked immunosorbent assay (ELISA) using a specific monoclonal antibody NCL-HGM-45M1 and polyclonal antiserum TEPA, respectively. Time response (0-72 hours) and doseresponse (1.5-150 ng/ml) studies were carried out. IL-6 and TNF-alpha stimulated MUC5AC and MUC5B mucin secretion in a time dependent manner, both in preconfluent and post-confluent cells, IL-6 (15 ng/ml and 20 ng/ml) produced a;low and prolonged stimulation of mucin secretion that persisted for 72 hours, with peak response at 24 hours after induction. The IL-6-mediated mucin secretion at 24 hours was concentration-dependent, with a maximal effect at 15 ng/ml, TNF-alpha (20 ng/ml) induced rapid stimulation of mucin secretion within the first 24 hours, with peak response at 7 hours after induction, IL-6 and TNF-alpha exposure significantly increased MUC5AC secretion, but not MUC5B secretion, Maximal levels of cytokine-induced mucin secretion were detected in pre-confluent cells that showed one and a half- and two-fold increases in MUC5AC secretion after IL-6 and TNF-alpha stimulation, respectively, in comparison with post-confluent cells. The results presented here suggest that IL-6 and TNF-alpha generate a differential up-regulation of mucin secretion and thus contribute to the expression of mucin genes in inflammatory responses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/02/20 alle ore 06:02:53