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Titolo:
Characterization of cytokine mRNA expression in tumour-infiltrating mononuclear cells and tumour cells freshly isolated from human colorectal carcinomas
Autore:
Csiszar, A; Szentes, T; Haraszti, B; Zou, WP; Emilie, D; Petranyi, G; Pocsik, E;
Indirizzi:
Natl Inst Haematol & Immunol, Dept Immunol, H-1113 Budapest, Hungary Natl Inst Haematol & Immunol Budapest Hungary H-1113 3 Budapest, Hungary Szent Imres Hosp, Dept Surg, Budapest, Hungary Szent Imres Hosp BudapestHungary es Hosp, Dept Surg, Budapest, Hungary Baylor Inst Immunol Res, Dallas, TX 75204 USA Baylor Inst Immunol Res Dallas TX USA 75204 nol Res, Dallas, TX 75204 USA Inst Paris Sud Cytokines, INSERM, U131, F-92140 Clamart, France Inst ParisSud Cytokines Clamart France F-92140 F-92140 Clamart, France
Titolo Testata:
EUROPEAN CYTOKINE NETWORK
fascicolo: 1, volume: 12, anno: 2001,
pagine: 87 - 96
SICI:
1148-5493(200103)12:1<87:COCMEI>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; ENDOTHELIAL GROWTH-FACTOR; HUMAN COLON-CARCINOMA; GENE-EXPRESSION; T-CELLS; INTERFERON-GAMMA; IN-SITU; LYMPHOCYTIC INFILTRATION; ANTIGEN EXPRESSION; IFN-GAMMA;
Keywords:
colorectal cancer; tumour-infiltrating lymphocytes; cytokines; gene expression; RT-PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Pocsik, E Natl Inst Haematol & Immunol, Dept Immunol, Daroczi Ut 24, H-1113 Budapest, Hungary Natl Inst Haematol & Immunol Daroczi Ut 24 Budapest Hungary H-1113
Citazione:
A. Csiszar et al., "Characterization of cytokine mRNA expression in tumour-infiltrating mononuclear cells and tumour cells freshly isolated from human colorectal carcinomas", EUR CYTOKIN, 12(1), 2001, pp. 87-96

Abstract

The in: situ function of tumour-infiltrating leukocytes (TIL) in human colorectal adenocarcinoma (CRC) is unclear, Local cytokine expression probablyregulates the anti-tumour immune response and tumour immune surveillance. We examined the distribution of mRNA for IFN-gamma, TNF-alpha, IL-10 and IL-4 in TIL, and tumour cells freshly isolated from 21 surgically removed primary CRC, using a semiquantitative RT-PCR, Lamina propria-infiltrating leukocytes (LPL) and epithelial cells from normal colon mucosa of 10 CRC patients served as negative controls. Median levels of IFN-gamma and TNF-alpha mRNA were higher in TIL than LPL (p = 0.0002 and 0.0001). IL-10 mRNA was generally observed in TIL and LPL, but no or very small amounts of IL-4 transcripts were detected in TIL and LPL, TNF-alpha and IL-10 mRNA were more abundant in colorectal tumour cells than in the normal epithelial cells (p = 0.0136 and 0,0036), The number of IFN-gamma transcripts in TIL correlated negatively (p = 0.039) and the number of TNF-alpha transcripts in tumour cells correlated positively with the Dukes' stages (p = 0.0147), Our results suggest that TIL are characterized by a type 1 (Th1/Tc1-like) pattern of cytokine expression and function as T cells land macrophages) in the local, cell-mediated antitumour immune response in early stages of CRC, Changes in IFN-gamma and TNF-alpha mRNA in TIL and tumour cells could be related to tumourprogress (e,g, by T cell anergy) or forming of metastases, respectively.

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Documento generato il 31/03/20 alle ore 18:14:34