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Titolo:
CENTRIFUGATION DOES NOT ALTER SPATIAL-DISTRIBUTION OF BEP4 MESSENGER-RNA IN PARACENTROTUS-LIVIDUS EGG
Autore:
COSTA C; RINALDI AM; ROMANCINO DP; CAVALCANTE C; VIZZINI A; DICARLO M;
Indirizzi:
CNR,IST BIOL SVILUPPO,VIA ARCHIRAFI 20 I-90123 PALERMO ITALY CNR,IST BIOL SVILUPPO I-90123 PALERMO ITALY UNIV PALERMO,DIPARTIMENTO BIOL CELLULARE & SVILUPPO I-90128 PALERMO ITALY
Titolo Testata:
FEBS letters
fascicolo: 2-3, volume: 410, anno: 1997,
pagine: 499 - 501
SICI:
0014-5793(1997)410:2-3<499:CDNASO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Keywords:
CENTRIFUGATION; A/V AXIS; LOCALIZED MESSENGER-RNA; CORTEX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
C. Costa et al., "CENTRIFUGATION DOES NOT ALTER SPATIAL-DISTRIBUTION OF BEP4 MESSENGER-RNA IN PARACENTROTUS-LIVIDUS EGG", FEBS letters, 410(2-3), 1997, pp. 499-501

Abstract

Paracentrotus lividus unfertilized eggs were centrifuged in a sucrosegradient, so to split each into two parts: a nucleated light fragmentand an anucleated heavy fragment. Northern blot analyses utilizing a bep4 probe as animal marker and H2A histone gene and 12S-mit RNA as controls indicate that the eggs are elongated along the animal-vegetal axis during centrifugation and thereafter split into an animal and a vegetal half, Treatment of the eggs with colchicine before centrifugation abolishes the animal localization of bep4 mRNA. (C) 1997 Federation of European Biochemical Societies.

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Documento generato il 01/10/20 alle ore 05:15:52