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Titolo:
Methylene blue as a novel electrochemical hybridization indicator
Autore:
Erdem, A; Kerman, K; Meric, B; Ozsoz, M;
Indirizzi:
Ege Univ, Fac Pharm, Dept Analyt Chem, TR-35100 Izmir, Turkey Ege Univ Izmir Turkey TR-35100 Dept Analyt Chem, TR-35100 Izmir, Turkey
Titolo Testata:
ELECTROANALYSIS
fascicolo: 3, volume: 13, anno: 2001,
pagine: 219 - 223
SICI:
1040-0397(200103)13:3<219:MBAANE>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
QUARTZ-CRYSTAL MICROBALANCE; HEPATITIS-B VIRUS; MODIFIED ELECTRODES; DICHROISM SPECTROSCOPY; METAL-COMPLEXES; NUCLEIC-ACIDS; DNA; BINDING; BIOSENSORS; OXIDATION;
Keywords:
hybridization indicator; methylene blue; DNA; DNA biosensors; ruthenium bipyridine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Ozsoz, M Ege Univ, Fac Pharm, Dept Analyt Chem, TR-35100 Izmir, Turkey EgeUniv Izmir Turkey TR-35100 lyt Chem, TR-35100 Izmir, Turkey
Citazione:
A. Erdem et al., "Methylene blue as a novel electrochemical hybridization indicator", ELECTROANAL, 13(3), 2001, pp. 219-223

Abstract

The interaction of double stranded DNA (dsDNA) and single stranded DNA (ssDNA) with Ru(II) complex with 2,2 ' -bipyridine ligand (Ru(bpy)(3)](2+)) and methylene blue (MB) were explored by using voltammetric and spectrophotometric methods. The electrochemistry of [Ru(bpy)(3)](2+) and MB was also investigated at a dsDNA-modified carbon paste electrode (CPE), a ssDNA-modified CPE and a bare CPE by means of differential pulse voltammetry (DPV), and cyclic voltammetry (CV) and the increased peak currents were observed, in respect to the order of electrodes. The structure of DNA was determined by the magnitudes of the voltammetric peaks of [Ru(bpy)(3)](2+) and MB. The hypochromicity of the visible absorption bands of [Ru(bpy)(3)](2+) and MB uponthe interaction with dsDNA and ssDNA was observed. Numerous factors affecting the DNA immobilization, indicator binding reactions and ionic strength were optimized to maximize the sensitivity and reduce the assay time.

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Documento generato il 23/01/21 alle ore 03:26:54