Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Loss of alpha 3 beta 1 integrin function results in an altered differentiation program in the mouse submandibular gland
Autore:
Menko, KS; Kreidberg, JA; Ryan, TT; Van Bockstaele, E; Kukuruzinska, MA;
Indirizzi:
Thomas Jefferson Univ, Dept Pathol Anat & Cell Biol, Philadelphia, PA 19107 USA Thomas Jefferson Univ Philadelphia PA USA 19107 hiladelphia, PA 19107 USA Harvard Univ, Sch Med, Dept Pediat, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 h Med, Dept Pediat, Boston, MA 02115 USA Childrens Hosp, Dept Med, Boston, MA 02115 USA Childrens Hosp Boston MA USA 02115 s Hosp, Dept Med, Boston, MA 02115 USA Boston Univ, Sch Med, Dept Biochem, Boston, MA 02118 USA Boston Univ Boston MA USA 02118 h Med, Dept Biochem, Boston, MA 02118 USA Boston Univ, Sch Dent Med, Dept Mol & Cell Biol, Boston, MA 02118 USA Boston Univ Boston MA USA 02118 ept Mol & Cell Biol, Boston, MA 02118 USA
Titolo Testata:
DEVELOPMENTAL DYNAMICS
fascicolo: 4, volume: 220, anno: 2001,
pagine: 337 - 349
SICI:
1058-8388(200104)220:4<337:LOA3B1>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPITHELIAL-CELL POLARITY; EPIDERMAL GROWTH-FACTOR; BASEMENT-MEMBRANE; SALIVARY-GLANDS; CYTOSKELETAL ORGANIZATION; BRANCHING MORPHOGENESIS; MONOCLONAL-ANTIBODIES; EXTRACELLULAR-MATRIX; ADHESION MOLECULES; RECEPTOR FUNCTION;
Keywords:
alpha 3 beta 1 integrin; laminin beta 1; fibronectin; alpha 5 integrin; E-cadherin; ERK; actin cytoskeleton;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Menko, KS Thomas Jefferson Univ, Dept Pathol Anat & Cell Biol, 571 Jefferson Alumni Hall,1020 Locust St, Philadelphia, PA 19107 USA Thomas Jefferson Univ 571 Jefferson Alumni Hall,1020 Locust St Philadelphia PA USA 19107
Citazione:
K.S. Menko et al., "Loss of alpha 3 beta 1 integrin function results in an altered differentiation program in the mouse submandibular gland", DEV DYNAM, 220(4), 2001, pp. 337-349

Abstract

Mammalian submandibular gland (SMG) development leads to the establishmentof highly organized secretory acinar and nonsecretory ductal epithelial cells. The ability of maturing salivary epithelial cells to attain their differentiated state has been shown to depend, in part, on interactions betweenextracellular matrix (ECM) proteins and their integrin receptors. In a search for key regulators of salivary cell lineage, we have studied alpha3 beta1 integrin, a receptor for the basement membrane protein laminin, by characterizing embryonic day 18 (E18) SMGs isolated from mice carrying a targeted mutation in the alpha3 integrin gene. Transmission electron microscopy studies showed that the mutant SMGs exhibited an aberrant differentiation phenotype with defects in the apical-basal polarity axis and in the basement membrane. Based on immunohistochemistry and Western blot analyses, the alpha3 beta1-deficient SMGs had altered expression and/or localization of several ECM and adhesive molecules, including laminin beta1, fibronectin, alpha5 integrin, and E-cadherin, These changes correlated with alterations in the activation state of Ras-extracellular signal-regulated kinase (ERK), as well as the expression and/or localization of Cdc42 and RhoA, two Rho GTPases that regulate the organization of the actin cytoskeleton. We conclude that alpha3 beta1 is required for normal salivary cell differentiation and that its absence affects multiple components of adhesive complexes and their associated signalling pathways. (C) 2001 Wiley-Liss, Inc.

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Documento generato il 06/04/20 alle ore 01:57:02