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Titolo:
Monoclonal antibodies to European flat oyster Ostrea edulis hemocytes: characterization and tissue distribution of granulocytes in adult and developing animals
Autore:
Xue, Q; Renault, T;
Indirizzi:
IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France IFREMER La Tremblade France F-17390 Pathol, F-17390 La Tremblade, France
Titolo Testata:
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
fascicolo: 3, volume: 25, anno: 2001,
pagine: 187 - 194
SICI:
0145-305X(200104)25:3<187:MATEFO>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
DICENTRARCHUS-LABRAX L; MUSSEL MYTILUS-EDULIS; RUDITAPES DECUSSATUS MOLLUSCA; CRASSOSTREA-GIGAS MOLLUSCA; FLOW CYTOMETRIC ANALYSIS; CYPRINUS-CARPIO L; ARGOPECTEN PURPURATUS; PHAGOCYTIC ABILITY; VIBRIO-ANGUILLARUM; LYMNAEA-STAGNALIS;
Keywords:
European flat oyster; Ostrea edulis; monoclonal antibody; hemocyte; granulocytes; tissue distribution; ontogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Renault, T IFREMER, Lab Genet & Pathol, BP 133, F-17390 La Tremblade, France IFREMER BP 133 La Tremblade France F-17390 a Tremblade, France
Citazione:
Q. Xue e T. Renault, "Monoclonal antibodies to European flat oyster Ostrea edulis hemocytes: characterization and tissue distribution of granulocytes in adult and developing animals", DEV COMP IM, 25(3), 2001, pp. 187-194

Abstract

Monoclonal antibodies specific for hemocyte sub-populations of the European flat oyster, Ostrea edulis, were prepared using separated granulocytes and hyalinocytes as antigen sources. Six monoclonal antibodies specific for hemocytes were selected. Five of them showed a specificity for more than onehemocyte type and one hybridoma produced a monoclonal antibody reacting specifically with granulocytes. At the ultrastructural level, this monoclonalantibody demonstrated epitopes principally in dense cytoplasmic granules of granulocytes. Western blotting analysis indicated that a peptide of 50 kDa was recognized by this antibody. It was therefore used to investigate granulocyte distribution and ontogenesis in European flat oysters using immunohistochemistry. Granulocytes were mostly observed in connective tissues in different organs. Their distribution pattern in digestive gland, mantle, gills and gonad may indicate different functional status. Morover, the absence of granulocytes in early larval stages can partly correspond to an immature immune system in oyster larvae. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 07:01:51