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Titolo:
Tissue-specific expression of two metallothionein genes in common carp during cadmium exposure and temperature shock
Autore:
Hermesz, E; Abraham, M; Nemcsok, J;
Indirizzi:
Univ Szeged, Fac Sci, Dept Biochem, H-6701 Szeged, Hungary Univ Szeged Szeged Hungary H-6701 , Dept Biochem, H-6701 Szeged, Hungary Bay Zoltan Fdn Appl Res, Inst Biotechnol, H-6727 Szeged, Hungary Bay Zoltan Fdn Appl Res Szeged Hungary H-6727 ol, H-6727 Szeged, Hungary
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
fascicolo: 3, volume: 128, anno: 2001,
pagine: 457 - 465
SICI:
1532-0456(200103)128:3<457:TEOTMG>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARASSIUS-CUVIERI; CYPRINUS-CARPIO; SALMO-GAIRDNERI; MESSENGER-RNA; RAINBOW-TROUT; COPPER LEVELS; HEAVY-METALS; ZINC; ISOFORMS; FISH;
Keywords:
cadmium treatment; carp; cold stress; kidney; liver; MT-1 mRNA; MT-2 mRNA; transcription;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Hermesz, E Univ Szeged, Fac Sci, Dept Biochem, POB 533, H-6701 Szeged, Hungary Univ Szeged POB 533 Szeged Hungary H-6701 6701 Szeged, Hungary
Citazione:
E. Hermesz et al., "Tissue-specific expression of two metallothionein genes in common carp during cadmium exposure and temperature shock", COMP BIOC C, 128(3), 2001, pp. 457-465

Abstract

Two metallothionein cDNA isoforms (MT-1 and MT-2) were isolated from carp (Cyprinus carpio) by RT-PCR. Sequence analysis of the cDNAs revealed two amino acid differences between the coding regions and markedly different 3'-untranslated ends. Gene-specific primers were selected and used in RT-PCR reactions to measure the basal MT-I and MT-2 mRNA levels and to follow the inducer-specific expression of MT genes in different tissues during in vivo studies. In the brain and muscle, the uninduced levels of the two MT mRNAs are similar. In the kidney and liver, the MT-I gene product predominated, while in the heart the relative expression levels of the two genes were opposite. Both the MT-I and MT-2 mRNA levels increased with Cd concentration in a time- and dose-dependent manner. The expression of MT-2, however, was more responsive to a high Cd concentration. In parallel with the induction of the MTs by Cd, we followed the accumulation of this metal in the kidney andliver. Although the Cd level was always higher in the kidney during treatment, the rate of accumulation was higher in the liver. Cold stress resultedin a significantly higher induction of MT-1 than of MT-2, while heat shockhad no effect on the expression of either gene. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 22:45:14