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Titolo:
Toxicity of coelomic fluid of the earthworm Eisenia foetida to vertebratesbut not invertebrates: probable role of sphingomyelin
Autore:
Kobayashi, H; Ohtomi, M; Sekizawa, Y; Ohta, N;
Indirizzi:
Toho Univ, Fac Sci, Dept Biomol Sci, Funabashi, Chiba 2748510, Japan Toho Univ Funabashi Chiba Japan 2748510 , Funabashi, Chiba 2748510, Japan Zenyaku Kogyo Co Ltd, Res Lab, Nerima Ku, Tokyo 1780062, Japan Zenyaku Kogyo Co Ltd Tokyo Japan 1780062 Nerima Ku, Tokyo 1780062, Japan
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
fascicolo: 3, volume: 128, anno: 2001,
pagine: 401 - 411
SICI:
1532-0456(200103)128:3<401:TOCFOT>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELOMIC FLUID; ANTIBACTERIAL PROTEINS; CAUSES CONTRACTION; BINDING-PROTEIN; FETIDA-ANDREI; LYSENIN; IDENTIFICATION; SPHINGOLIPIDS; PURIFICATION; AGGLUTININS;
Keywords:
coelomic fluid; earthworm; lysenin; phylogeny; sphingomyelin; toxicity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Kobayashi, H 3-16-17 Kamisaginomiya,Nakano Ku, Tokyo 1650031, Japan 3-16-17 Kamisaginomiya,Nakano Ku Tokyo Japan 1650031 Japan
Citazione:
H. Kobayashi et al., "Toxicity of coelomic fluid of the earthworm Eisenia foetida to vertebratesbut not invertebrates: probable role of sphingomyelin", COMP BIOC C, 128(3), 2001, pp. 401-411

Abstract

The coelomic fluid (CF) of the earthworm Eisenia foetida exhibits a wide variety of biological activities. We found that the CF was not toxic to 32 species, belonging to seven invertebrate phyla, almost all in aquatic adultsand larvae exposed to CF. Eleven teleostean species tested died in 0.2-1% CF mostly between 10 and 120 min and the effects were dose-dependent. Tadpoles of the toad Bufo japonicus formosus died in 0.4-2% CF between 80 and 225 min depending upon size, with larger tadpoles surviving longer. Before dying, all experimental tadpoles developed curled and shrunken tails. The Okinawa tree lizard, soft-shelled turtle, Japanese quail, mouse and rat all died after i.v. injection of CF (above 20 mul/kg). Thus, CF was not toxic to invertebrates, but toxic to vertebrates. After heating, CF lost its toxicity to fish, tadpoles and mice. Both CF and lysenin incubated with sphingomyelin-liposomes (SM-liposomes) were no longer toxic, suggesting the involvement of SM in the toxicity. Lysenin, which is a constituent of CF and known to bind specifically to sphingomyelin, exhibited toxicity similar to that ofCF. Thus, lysenin in CF is probably responsible for the toxic effects of CF by binding to SM in vertebrate tissues. The bodies of invertebrates mightcontain little or no SM, while those of vertebrates do contain SM. The coelomic fluid of the earthworm Pheretima communissima has no toxicity to mouse. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 22:13:48