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Titolo:
Crystallization of RNA
Autore:
Holbrook, SR; Holbrook, EL; Walukiewicz, HE;
Indirizzi:
Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Phys Biosci Div, Dept Biol Struct, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 Struct, Berkeley, CA 94720 USA
Titolo Testata:
CELLULAR AND MOLECULAR LIFE SCIENCES
fascicolo: 2, volume: 58, anno: 2001,
pagine: 234 - 243
SICI:
1420-682X(200102)58:2<234:COR>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
5S RIBOSOMAL-RNA; PHENYLALANINE TRANSFER-RNA; CRYSTAL-STRUCTURE; THERMUS-FLAVUS; ANGSTROM RESOLUTION; CAPILLARY ELECTROPHORESIS; 3-DIMENSIONAL STRUCTURE; PRELIMINARY DIFFRACTION; HAMMERHEAD RIBOZYME; TERTIARY STRUCTURE;
Keywords:
RNA crystallization; X-ray crystallography; RNA crystal structure; sparse matrix crystallization; X-ray diffraction;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
67
Recensione:
Indirizzi per estratti:
Indirizzo: Holbrook, SR Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Phys Biosci Div, Dept Biol Struct, Berkeley, CA 94720 USA Univ Calif Berkeley BerkeleyCA USA 94720 eley, CA 94720 USA
Citazione:
S.R. Holbrook et al., "Crystallization of RNA", CELL MOL L, 58(2), 2001, pp. 234-243

Abstract

Even as the number of RNA structures determined and under study multiplies, the critical step in X-ray diffraction analysis, growth of single well-ordered crystals, remains at the boundary between art and science. Recent advances in methods of RNA synthesis, purification, and characterization, as well as empirical and technical improvements in crystallization techniques, the development of cryo-crystallography, and the wider availability of bright, tunable, X-rays from synchrotron sources are improving the chances of obtaining RNA crystals suitable for X-ray structural analysis. In this review, we summarize the current status of the design, preparation, purification, and analysis of RNA for crystallization and describe the latest approaches to obtaining diffraction-quality crystals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 13:32:21