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Titolo:
Phenotypic and functional characterization of human thymic stromal cell lines
Autore:
Wakkach, A; Chastre, E; Bruand, C; Cohen-Kaminsky, S; Emami, S; Gespach, C; Berrih-Aknin, S;
Indirizzi:
Hop Marie Lannelongue, CNRS ESA 8078, Lab Physiol Thym, F-92350 Le PlessisRobinson, France Hop Marie Lannelongue Le Plessis Robinson France F-92350obinson, France Hop St Antoine, INSERM U482, F-75571 Paris, France Hop St Antoine Paris France F-75571 , INSERM U482, F-75571 Paris, France
Titolo Testata:
CELLULAR AND MOLECULAR BIOLOGY
fascicolo: 1, volume: 47, anno: 2001,
pagine: 167 - 178
SICI:
0145-5680(200102)47:1<167:PAFCOH>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLASS-II GENES; MYOID CELLS; EPITHELIAL-CELLS; ACETYLCHOLINE-RECEPTOR; MYASTHENIA-GRAVIS; EXPRESSION; ESTABLISHMENT; REPERTOIRE; SELECTION;
Keywords:
human thymus; epithelial cell lines; myoid cell line; cytokine; chemokine; MHC antigens; LFA-3; ICAM-1; acetylcholine receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Berrih-Aknin, S Hop Marie Lannelongue, CNRS ESA 8078, Lab Physiol Thym, 133 Ave Resistance, F-92350 Le Plessis Robinson, France Hop Marie Lannelongue133 Ave Resistance Le Plessis Robinson France F-92350
Citazione:
A. Wakkach et al., "Phenotypic and functional characterization of human thymic stromal cell lines", CELL MOL B, 47(1), 2001, pp. 167-178

Abstract

To establish new tools for studying human thymic stromal cells, we transfected adherent cells from a human postnatal thymus using a plasmid encoding SV40 large T antigen. Among the cell lines obtained, we characterized four epithelial cell lines (LT-TEC1 to LT-TEC4) and one thymic myoid cell line (MITC). Several morphological, functional and phenotypic differences were observed between these 2 cell types. Epithelial cells were heterogeneous and larger than myoid cells. Untreated LT-TEC lines expressed MHC class I, ICAM-1 and LFA-3 antigens and not MHC class II antigens, similarly to primary thymic epithelial cells (PTEC), while MITC line expressed only class I and LFA-3 antigens. After IFN-gamma treatment, MHC class II and ICAM-1 antigens were markedly upregulated in LT-TEC lines but not in MITC, indicating the absence or a dysfunction of regulatory factors in MITC line. Myoid cells expressed mRNA for all the subunits of the acetylcholine receptor (AChR) whileepithelial cells expressed only the alpha, beta and epsilon subunits. Strikingly, LT-TEC produced much more C-C chemokines and IL-6 than MITC cells, while these latter produced higher levels of IL-8 and TNF-alpha. Altogether, these results reveal phenotypic and functional differences between these two stromal cell types, suggesting a potential involvement of myoid cells in the thymic function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 12:06:17