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Titolo:
The ''early'' CD4(+)CD8(+) stage of thymocyte differentiation hallmarks the end of a strong positive correlation between extracellular matrix receptor expression and protein tyrosine phosphorylation
Autore:
Dalmau, SR; Freitas, CS; Savino, W;
Indirizzi:
NCI, Basic Res Ctr, Div Expt Med, BR-20230130 Rio De Janeiro, Brazil NCI Rio De Janeiro Brazil BR-20230130 BC-20230130 Rio De Janeiro, Brazil Univ State Rio de Janeiro, Inst Biol, Dept Biochem, Rio De Janeiro, BrazilUniv State Rio de Janeiro Rio De Janeiro Brazil Rio De Janeiro, Brazil Oswaldo Cruz Fdn, Inst Oswaldo Cruz, Dept Immunol, Lab Thymus Res, Rio De Janeiro, Brazil Oswaldo Cruz Fdn Rio De Janeiro Brazil ymus Res, Rio De Janeiro, Brazil
Titolo Testata:
CELLULAR AND MOLECULAR BIOLOGY
fascicolo: 1, volume: 47, anno: 2001,
pagine: 75 - 86
SICI:
0145-5680(200102)47:1<75:T'CSOT>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
FOCAL ADHESION KINASE; T-CELL DEVELOPMENT; THYMIC STROMAL CELLS; REGULATED EXPRESSION; INTRATHYMIC SIGNALS; IRRADIATION LEADS; MURINE THYMOCYTES; EPITHELIAL-CELLS; FETAL THYMOCYTES; MATURATION STAGE;
Keywords:
phosphotyrosine; integrins; L-selectin; radiation; thymocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Dalmau, SR NCI, Basic Res Ctr, Div Expt Med, Praca Cruz Vermelha 23,6 Andar, BR-20230130 Rio De Janeiro, Brazil NCI Praca Cruz Vermelha 23,6 Andar Rio De Janeiro Brazil BR-20230130 BC
Citazione:
S.R. Dalmau et al., "The ''early'' CD4(+)CD8(+) stage of thymocyte differentiation hallmarks the end of a strong positive correlation between extracellular matrix receptor expression and protein tyrosine phosphorylation", CELL MOL B, 47(1), 2001, pp. 75-86

Abstract

We used irradiation-induced thymic regression/reconstitution to study phosphotyrosine (PTyr) levels and expression of extracellular matrix receptors in thymocyte subsets by flow cytometry. High PTyr levels (PTyr(hi)) characterized cells from the CD4(-)CD8(-)(DN)CD25(in/hi) to the "early" CD4(+)CD8()(DP)CD25(-) stage. Correlation indexes (R) between the percentages of these PTyr(hi) cells and cells with up-regulated expression of alpha4 integrin(alpha4(hi)) were strongly positive (R= 0.91, P= 0.002, for DN; R= 0.98, P= 0.0001 for DP). At the "early" DP stage, R between PTyr(hi) cells and cells with up-regulated expression of alpha5 integrin and L-selectin (alpha5(hi) and L-sel(hi)) also rendered strongly positive (R>0.95, p<0.0003). "Late" expanding DP cells exhibited intermediate PTyr levels (PTyr(hi)), associated with a down-regulation of the adhesion receptors assessed. Triple-labeling suggested that in most early CD3(-/lo) cells, <alpha>4(hi) and alpha5(hi), but not L-sel(hi) expression preceded a PTyr(hi) content. CD3(in/hi)-enriched CD8(+) cells were also PTyr(hi), but conversely to the immature onesexhibited a tendency for a negative R between PTyr(hi) and alpha4(hi) (R= -0.93, P= 0.067, n= 4) or alpha5(hi) cells (R= -0.77, P= 0.23, n= 4). CD4(+) cells were either PTyr(hi) or PTyr(hi), exhibiting a tendency for a positive R (R= 0.59, P= 0.124, n= 8) between PTyr(hi) and L-sel(hi) cells only. In conclusion, our results associate an up-regulation of alpha4 and alpha5 chains expression with PTyr(hi) levels and, as elsewhere published, with increased adhesion to fibronectin up to the "early" DP stage, but not afterwards.

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Documento generato il 01/04/20 alle ore 11:26:18