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Titolo:
Characterization of P2 receptors in thymic epithelial cells
Autore:
Bisaggio, RD; Nihei, OK; Persechini, PM; Savino, W; Alves, LA;
Indirizzi:
Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Dept Imunol, Lab Pesquisas Timo,Rio De Janeiro, Brazil Fundacao Oswaldo Cruz Rio De Janeiro Brazil Timo,Rio De Janeiro, Brazil Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Membranas Excitaveis, BR-21941 Rio De Janeiro, Brazil Univ Fed Rio de Janeiro Rio De Janeiro Brazil BR-21941 e Janeiro, Brazil Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Imunobiofis,BR-21941 Rio De Janeiro, Brazil Univ Fed Rio de Janeiro Rio De Janeiro Brazil BR-21941 e Janeiro, Brazil
Titolo Testata:
CELLULAR AND MOLECULAR BIOLOGY
fascicolo: 1, volume: 47, anno: 2001,
pagine: 19 - 31
SICI:
0145-5680(200102)47:1<19:COPRIT>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXTRACELLULAR-MATRIX COMPONENTS; P-2Z PURINERGIC RECEPTORS; NUCLEOTIDE RECEPTOR; INTERFERON-GAMMA; PHOSPHOLIPASE-D; ATP; ACTIVATION; CALCIUM; MICROENVIRONMENT; MACROPHAGES;
Keywords:
extracellular ATP; purinergic receptors; thymic epithelial cells; thymic Nurse cells; calcium; P2X(7); P2Y(2); permeabilization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Alves, LA Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Dept Imunol, Lab Pesquisas Timo,Rio De Janeiro, Brazil Fundacao Oswaldo Cruz Rio De Janeiro Brazil e Janeiro, Brazil
Citazione:
R.D. Bisaggio et al., "Characterization of P2 receptors in thymic epithelial cells", CELL MOL B, 47(1), 2001, pp. 19-31

Abstract

The presence of P2 receptors was investigated in three distinct preparations of murine thymic epithelial cells (TEC): 2BH4 murine cell line, IT45-R1 rat cell line, and a primary murine cell derived fi om the Nurse cell lympho-epithelial complex. In all preparations, application of ATP to the extracellular milieu triggered intracellular calcium signals indicating the presence of P2 receptor(s) in these cells. After an initial peak of calcium concentration, a plateau phase that could last more than 10 min was frequently observed. Ion replacement and channel blockage experiments indicated that the initial peak was associated with the release of calcium fi om intracellular stores, while the plateau phase was associated with an influx hem the extracellular medium. ATP and UTP induced similar calcium signals, suggesting the presence of P2Y(2) receptors in all three cell types. The murine 2BH4cells also expressed P2X(7)/P-2Z receptor, since under exposure to millimolar concentrations of ATP, a continuous rise in intracellular calcium concentration was observed and their plasma membranes became permeabilized to the fluorescent dyes Lucifer yellow and ethidium bromide. In addition, this permeabilization phenomenon was blocked by the P-2z-specific antagonist, oxidized ATP. RT-PCR assays confirmed the presence of mRNAs for the P2Y(2) molecule in all TEC, while mRNA for the P2X(7) molecule was detected only in 2BH4 cells. Our data indicate that P2Y(2) purinergic receptors are widely expressed by thymic epithelial cells, whereas the expression of the P2X(7) receptor appears to be more restricted, raising the possibility that its expression is related only to a particular epithelial microenvironment within the thymus.

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Documento generato il 02/12/20 alle ore 05:35:05