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Titolo:
Prolonged postlesion transplantation delay adversely influences survival of both homotopic and heterotopic fetal hippocampal cell grafts in kainate-lesioned CA3 region of adult hippocampus
Autore:
Zaman, V; Turner, DA; Shetty, AK;
Indirizzi:
Duke Univ, Med Ctr, Div Neurosurg, Dept Surg Neurosurg & Neurobiol, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 Neurosurg & Neurobiol, Durham, NC 27710 USA Vet Adm Med Ctr, Med Res Serv, Durham, NC 27705 USA Vet Adm Med Ctr Durham NC USA 27705 r, Med Res Serv, Durham, NC 27705 USA Vet Adm Med Ctr, Surg Neurosurg Serv, Durham, NC 27705 USA Vet Adm Med Ctr Durham NC USA 27705 Neurosurg Serv, Durham, NC 27705 USA
Titolo Testata:
CELL TRANSPLANTATION
fascicolo: 1, volume: 10, anno: 2001,
pagine: 41 - 52
SICI:
0963-6897(200101/02)10:1<41:PPTDAI>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST GROWTH-FACTOR; TEMPORAL-LOBE EPILEPSY; CENTRAL-NERVOUS-SYSTEM; FACTOR MESSENGER-RNA; KAINIC ACID; RAT HIPPOCAMPUS; INDUCED NEURODEGENERATION; HUNTINGTONS-DISEASE; NEUROTROPHIC FACTOR; ASTROCYTES EXPRESS;
Keywords:
5'-bromodeoxyuridine; graft cell survival; graft integration; hippocampus; kainic acid; neural transplantation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Shetty, AK Duke Univ, Med Ctr, Div Neurosurg, Dept Surg Neurosurg & Neurobiol, Box 3807, Durham, NC 27710 USA Duke Univ Box 3807 Durham NC USA 27710 07, Durham, NC 27710 USA
Citazione:
V. Zaman et al., "Prolonged postlesion transplantation delay adversely influences survival of both homotopic and heterotopic fetal hippocampal cell grafts in kainate-lesioned CA3 region of adult hippocampus", CELL TRANSP, 10(1), 2001, pp. 41-52

Abstract

Fetal hippocampal CA3 cell grafts exhibit dramatically enhanced survival when transplanted at an early postlesion delay of 4 days into the lesioned CA3 region of adult hippocampus. However, survival of these homotopic graftsfollowing placement at late postlesion time points when the host milieu isconsiderably less receptive to grafts is unknown. We hypothesize that an extended postlesion delay at the rime of grafting will lead to significant diminution in cell survival of both homotopic and heterotopic fetal transplants grafted to lesioned adult CNS. We quantitatively investigated absolute cell survival of 5'-bromodroxyuridine-labeled fetal hippocampal CA3 and CA1cell grafts, following transplantation into the lesioned CA3 region of adult rat hippocampus, at a delay of 45 days after a unilateral intracerebroventricular administration of kainic acid (KA). Survival of these grafts was also analyzed in intact CA3 of the hippocampus contralateral to KA administration for comparison. In lesioned CR3 region, CA3 (homotopic) and CA1 (heterotopic) grafts exhibited comparable but only moderate survival, with a recovery of only 21-31% of injected cells. Cell survival in these grafts intolesioned hippocampus was similar to survival of grafts placed into the contralateral intact CA3 region. These results are in sharp contrast to increased graft survival measured following transplants performed at 4 days postlesion. in such grafts placed early, there was both a significantly higher cell survival than grafts placed into the intact CA3 region and also a characteristic differential survival based on graft cell specificity to the lesioned CA3 region (Zaman et al., Exp. Neurol., 161:535-561, 2000). Thus, the enhanced conduciveness of lesioned CA3 region for survival of homotopic CA3cell grafts observed at 4 days postlesion wanes by 45 days postlesion to that of intact CA3 region, in spite of residual loss of CA3 neurons with thelesion. Strategies that considerably augment graft cell survival may therefore be critical for optimal integration of fetal grafts into the adult CNSat late postlesion time points.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 14:37:16