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Titolo:
Colonisation of prosthetic grafts by immunocompetent cells in a sheep model
Autore:
Bobryshev, YV; Inder, SJ; Cherian, SM; Lord, RSA; Ao, PY; Hawthorne, WJ; Fletcher, JP;
Indirizzi:
Univ New S Wales, St Vincents Hosp, Professorial Surg Unit, Sydney, NSW 2010, Australia Univ New S Wales Sydney NSW Australia 2010 t, Sydney, NSW 2010, Australia Univ Sydney, Westmead Hosp, Dept Surg, Sydney, NSW 2145, Australia Univ Sydney Sydney NSW Australia 2145 t Surg, Sydney, NSW 2145, Australia
Titolo Testata:
CARDIOVASCULAR SURGERY
fascicolo: 2, volume: 9, anno: 2001,
pagine: 166 - 176
SICI:
0967-2109(200104)9:2<166:COPGBI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTIMAL HYPERPLASIA; DENDRITIC CELLS; VASCULAR PROSTHESES; IN-VIVO; DACRON; ARTERIES; POLYMERS; CLOSURE;
Keywords:
synthetic grafts; inflammation; immunocompetent cells; dendritic cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Bobryshev, YV Univ New S Wales, St Vincents Hosp, Professorial Surg Unit, Sydney, NSW 2010, Australia Univ New S Wales Sydney NSW Australia 2010 2010, Australia
Citazione:
Y.V. Bobryshev et al., "Colonisation of prosthetic grafts by immunocompetent cells in a sheep model", CARDIOV SUR, 9(2), 2001, pp. 166-176

Abstract

The present study examined the distribution of immunocompetent cells in synthetic vascular grafts in an experimental sheep model. Sixty-two adult Merino sheep underwent synthetic patch closure of a longitudinal arteriotomy in the left common carotid artery. The synthetic patch materials used were gelatin sealed Dacron (n = 10), fluoropassivated Dacron (n = 10), Fluoropassiv (n = 12), polyurethane (n = 10), expanded polytetrafluoroethylene (n = 10) and carbon-lined expanded polytetrafluoroethylene (n = 10), The sheep were sacrificed after four weeks when the prosthetic patches were harvested and fixed in 10% neutral buffered formalin. Transverse sections were taken along the graft and paraffin embedded. Serial sections were stained with cell type specific antibodies to identify T-lymphocytes (CD3(+)), dendritic cells (S-100(+)), endothelial cells (von Willebrand factor(+)) and smooth muscle cells (smooth muscle alpha-actin(+)), All six graft types contained CD3(+) and S-100(+) cells in the neointima, within the synthetic matrix and inthe perigraft layer. Three different tissue responses to synthetic materials were observed and the grafts were classified accordingly into three groups: (1) gelatin sealed Dacron, fluoropassivated Dacron and Fluoropassiv; (2) expanded polytetrafluoroethylene and carbon-lined expanded polytetrafluoroethylene; (3) polyurethane. The three synthetic materials in Group 1 showed almost identical reactions with least accumulation of immunocompetent cells within the synthetic material but greater accumulation of immunoinflammatory infiltrates in the perigraft vascular tissue. In this group, new vessels penetrated into the synthetic material and there was prominent formationof foreign body (giant) cells, Group 2 showed greater accumulation of immunocompetent cells within the synthetic material itself but only sparse immune-inflammatory infiltrates in the perigraft tissue. Group 3 showed a high degree of inflammatory response within both the synthetic material and the perigraft vascular tissue, These observations demonstrate that immunocompetent cells colonise the synthetic matrix of grafts and accumulate in the perigraft tissue, but inflammatory responses vary in different graft types. (C) 2001 The International Society for Cardiovascular Surgery. Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 12:27:15