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Titolo:
Loss of cyclin D2 expression in the majority of breast cancers is associated with promoter hypermethylation
Autore:
Evron, E; Umbricht, CB; Korz, D; Raman, V; Loeb, DM; Niranjan, B; Buluwela, L; Weitzman, SA; Marks, J; Sukumar, S;
Indirizzi:
Johns Hopkins Univ, Sch Med, Dept Oncol, Baltimore, MD 21231 USA Johns Hopkins Univ Baltimore MD USA 21231 Oncol, Baltimore, MD 21231 USA Johns Hopkins Univ, Sch Med, Dept Surg, Baltimore, MD 21231 USA Johns Hopkins Univ Baltimore MD USA 21231 t Surg, Baltimore, MD 21231 USA Johns Hopkins Univ, Sch Med, Dept Radiol, Baltimore, MD 21231 USA Johns Hopkins Univ Baltimore MD USA 21231 Radiol, Baltimore, MD 21231 USA Univ London Imperial Coll Sci Technol & Med, Sch Med, Div Med, London W12 0NN, England Univ London Imperial Coll Sci Technol & Med London England W12 0NN gland Northwestern Univ, Sch Med, Chicago, IL 60611 USA Northwestern Univ Chicago IL USA 60611 iv, Sch Med, Chicago, IL 60611 USA Duke Univ, Med Ctr, Durham, NC 27710 USA Duke Univ Durham NC USA 27710Duke Univ, Med Ctr, Durham, NC 27710 USA
Titolo Testata:
CANCER RESEARCH
fascicolo: 6, volume: 61, anno: 2001,
pagine: 2782 - 2787
SICI:
0008-5472(20010315)61:6<2782:LOCDEI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-CYCLE; GENE-EXPRESSION; METHYLATION STATUS; D1 PROTEIN; PROGRESSION; OVEREXPRESSION; GROWTH; MYC; CARCINOMA; CHROMATIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Sukumar, S Johns Hopkins Oncol Ctr, BBCRB, Room 410,1650 Orleans St, Baltimore, MD 21231 USA Johns Hopkins Oncol Ctr Room 410,1650 Orleans St Baltimore MD USA 21231
Citazione:
E. Evron et al., "Loss of cyclin D2 expression in the majority of breast cancers is associated with promoter hypermethylation", CANCER RES, 61(6), 2001, pp. 2782-2787

Abstract

Cyclin D2 is a member of the D-type cyclins, implicated in cell cycle regulation, differentiation, and malignant transformation. It was noted previously that cyclin D2 is not expressed in the majority of breast cancer cell lines, whereas abundant expression was detected in finite life span human mammary epithelial cells. By reverse transcription-PCR and Western blot analysis, we extended this finding to primary breast carcinomas and show that the majority of these tumors lack expression of cyclin D2 mRNA (18 of 24) andprotein (10 of 13), In contrast, both luminal and myoepithelial subpopulations of normal breast tissues expressed cyclin D2. Hypermethylation of the CpG island in the promoter was detected by methylation-specific PCR in nearly half of the breast cancers (49 of 106) and was associated with silencingof cyclin D2 gene expression. Promoter hypermethylation was also detected in ductal carcinoma in situ, suggesting that loss of cyclin D2 expression is an early event in tumorigenesis. Our results suggest that loss of cyclin D2 expression is associated with the evolution of breast cancer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 03:21:07