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Titolo:
FLRF, a novel evolutionarily conserved RING finger gene, is differentiallyexpressed in mouse fetal and adult hematopoietic stem cells and progenitors
Autore:
Abdullah, JM; Li, XY; Nachtman, RG; Jurecic, R;
Indirizzi:
Univ Miami, Sch Med, Dept Microbiol & Immunol, Miami, FL 33136 USA Univ Miami Miami FL USA 33136 pt Microbiol & Immunol, Miami, FL 33136 USA
Titolo Testata:
BLOOD CELLS MOLECULES AND DISEASES
fascicolo: 1, volume: 27, anno: 2001,
pagine: 320 - 333
SICI:
1079-9796(200101)27:1<320:FANECR>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUPPRESSION SUBTRACTIVE HYBRIDIZATION; TRANSCRIPTION FACTORS; T-CELL; LYMPHOCYTE DEVELOPMENT; PROTEIN LMO2; BONE-MARROW; LEUKEMIA; ENRICHMENT; IDENTIFICATION; SEQUENCES;
Keywords:
differential gene expression; hematopoietic stem cells; hematopoietic progenitors; RING finger domain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Jurecic, R Univ Miami, Sch Med, Dept Microbiol & Immunol, R-138,1600 NW 10th Ave, Miami, FL 33136 USA Univ Miami R-138,1600 NW 10th Ave Miami FL USA 33136 33136 USA
Citazione:
J.M. Abdullah et al., "FLRF, a novel evolutionarily conserved RING finger gene, is differentiallyexpressed in mouse fetal and adult hematopoietic stem cells and progenitors", BL CELL M D, 27(1), 2001, pp. 320-333

Abstract

Through differential screening of mouse hematopoietic stem cell CHSC) and progenitor subtracted cDNA libraries we have identified a HSC-specific transcript that represents a novel RING finger gene, named FLRF (fetal liver ring finger). FLRF represent a novel evolutionarily highly conserved RING finger gene, present in Drosophila, zebrafish, Xenopus, mouse, and humans. Full-length cDNA clones for mouse and human gene encode an identical protein of 317 amino acids with a C3HC3 RING finger domain at the amino terminus, During embryonic hematopoiesis FLRF is abundantly transcribed in mouse fetal liver HSC (Sca-1(+) c-kit(+) AA4.1(+)Lin(-) cells), but is not expressed inprogenitors (AA4.1(-)). In adult mice FLRF is not transcribed in a highly enriched population of bone marrow HSC (Rh-123(low)Sca-1(+)c-kit(+) Lin(-) cells). Its expression is upregulated in a more heterogeneous population ofbone marrow HSC (Lin(-)Sca-1(+) cells), downregulated as they differentiate into progenitors (Lin(-)Sca-1(-) cells), and upregulated as progenitors differentiate into mature lymphoid and myeloid cell types. The human FLRF gene that spans a region of at least 12 kb and consists of eight exons was localized to chromosome 12q13, a region with frequent chromosome aberrations associated with multiple cases of acute myeloid leukemia and non-Hodgkin's lymphoma. The analysis of the genomic sequence upstream of the first exon in the mouse and human FLRF gene has revealed that both putative promoters contain multiple putative binding sites for several hematopoietic (GATA-1, GATA-2, GATA-3, Ikaros, SCL/Tal-1, AML1, MZF-1, and Lmo2) and other transcription factors, suggesting that mouse and human FLRF expression could be regulated in a developmental and cell-specific manner during hematopoiesis. Evolutionary conservation and differential expression in fetal and adult HSC and progenitors suggest that the FLRF gene could play an important role in HSC/progenitor cell lineage commitment and differentiation and could be involved in the etiology of hematological malignancies. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:35:47