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Titolo:
Hematopoietic stem cell transplantation in Utero produces sheep-goat chimeras
Autore:
Oppenheim, SM; Muench, MO; Gutierrez-Adan, A; Moyer, AL; BonDurant, RH; Rowe, JD; Anderson, GB;
Indirizzi:
Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 s, Dept Anim Sci, Davis, CA 95616 USA Univ Calif San Francisco, Fetal Treatment Ctr, San Francisco, CA 94122 USAUniv Calif San Francisco San Francisco CA USA 94122 ancisco, CA 94122 USA Univ Calif Davis, Dept Populat Hlth & Reprod, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 at Hlth & Reprod, Davis, CA 95616 USA
Titolo Testata:
BLOOD CELLS MOLECULES AND DISEASES
fascicolo: 1, volume: 27, anno: 2001,
pagine: 296 - 308
SICI:
1079-9796(200101)27:1<296:HSCTIU>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN FETAL LIVER; SEVERE COMBINED IMMUNODEFICIENCY; IN-UTERO; INUTERO TRANSPLANTATION; BONE-MARROW; T-CELLS; TOLERANCE; ENGRAFTMENT; EXPRESSION; MICE;
Keywords:
HSC transplantation; hematopoietic chimerism; sheep-goat chimera;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Anderson, GB Univ Calif Davis, Dept Anim Sci, 1 Shields Ave, Davis, CA 95616 USA Univ Calif Davis 1 Shields Ave Davis CA USA 95616 A 95616 USA
Citazione:
S.M. Oppenheim et al., "Hematopoietic stem cell transplantation in Utero produces sheep-goat chimeras", BL CELL M D, 27(1), 2001, pp. 296-308

Abstract

Both allogeneic and xenogeneic hematopoietic chimera models have been developed, including fetal sheep models that demonstrated high levels of stable, multilineage engraftment created by in utero hematopoietic stem cell transplantation. The aim of this study was to test the efficacy of in utero transplantation to create xenogeneic sheep-goat hematopoietic chimeras. Fetal liver cells and T-cell-depleted adult bone marrow were tested as sources ofhematopoietic stem cells. Donor cells were injected intraperitoneally into130 recipient fetuses between 49 and 62 days of gestation. Groups 1 and 2 received crude fetal liver cell preparations. Group 3 received fetal liver cells that were incubated overnight in a phytohemagglutinin-stimulated lymphocyte-conditioned medium (PHA-LCM). In Group 4, hematopoietic stem cells were concentrated by using additional density separations. Group 5 fetal recipients received low-density, T-cell-depleted adult bone marrow cells, in Group 1, fetuses were accessed via hysterotomy. Hematopoietic stem cells were injected into Groups 2, 3, 4, and 5 without cutting through the uterine wall. Fetal survival in the five groups ranged from 56 to 100%. The percentage of chimeras from injected fetuses ranged from 43 to 92% by FAGS and PCR analyses; however, levels of chimerism were low (<1%). The highest rates ofchimerism were found among recipients of low-density fetal liver cells. Despite the pre-immunocompetent status of the fetal recipients and the genetic similarities between sheep and goats, high levels of engraftment were notobserved. The consistently low levels of chimerism observed in this study,as well as the poor results recently reported by others using these procedures, indicate that significant barriers exist to transplanting hematopoietic stem cells in utero. <(c)> 2001 Academic Press.

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Documento generato il 06/04/20 alle ore 00:10:53