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Titolo:
Identification and validation of candidate Myb target genes
Autore:
Bartley, PA; Lutwyche, JK; Gonda, TJ;
Indirizzi:
Inst Med & Vet Sci, Hanson Ctr Canc Res, Adelaide, SA 5000, Australia InstMed & Vet Sci Adelaide SA Australia 5000 delaide, SA 5000, Australia Inst Med & Vet Sci, Div Human Immunol, Adelaide, SA 5000, Australia Inst Med & Vet Sci Adelaide SA Australia 5000 delaide, SA 5000, Australia
Titolo Testata:
BLOOD CELLS MOLECULES AND DISEASES
fascicolo: 2, volume: 27, anno: 2001,
pagine: 409 - 415
SICI:
1079-9796(200103)27:2<409:IAVOCM>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-MYB; HEMATOPOIETIC-CELLS; BINDING-SITES; MYELOID CELLS; DIFFERENTIATION; TRANSCRIPTION; PROMOTER; ONCOPROTEIN; REPRESSION; EXPRESSION;
Keywords:
Myb; transformation; target genes; RDA; cDNA array; Northern blotting;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Gonda, TJ Inst Med & Vet Sci, Hanson Ctr Canc Res, Frome Rd, Adelaide, SA 5000, Australia Inst Med & Vet Sci Frome Rd Adelaide SA Australia 5000 Australia
Citazione:
P.A. Bartley et al., "Identification and validation of candidate Myb target genes", BL CELL M D, 27(2), 2001, pp. 409-415

Abstract

While a considerable number of candidate Myb target genes have been reported to date, most of these are likely to play little or no role in transformation by myb oncogenes. Here we have used a conditionally myb-transformed myeloid cell line (ERMYB) to further examine Myb regulation of one candidatetarget gene-c-myc-that has the potential to affect cell proliferation. It was found that the major influence on c-myc expression was the presence of cytokine (GM-CSF) rather than Myb activity. We also describe the application of PCR-based subtractive hybridization and low-density cDNA array screening, in conjunction with the ERMYB line, to the identification of additionalMyb target genes. Preliminary identification of a number of candidates is reported; these include myeloperoxidase, which is known to have essential Myb-binding sites in its regulatory region. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 21:43:42