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Titolo:
Efficient in vitro micropropagation and regeneration of Humulus lupulus onlow sugar, starch-Gelrite media
Autore:
Smykalova, I; Ortova, M; Lipavska, H; Patzak, J;
Indirizzi:
Czech Univ Agr, Fac Agron, Lab Tissue Culture, CZ-16521 Prague 6, Czech Republic Czech Univ Agr Prague Czech Republic 6 CZ-16521 Prague 6, Czech Republic Charles Univ, Fac Sci, Dept Plant Physiol, CZ-12844 Prague, Czech RepublicCharles Univ Prague Czech Republic CZ-12844 12844 Prague, Czech Republic Hop Res Inst Co Ltd, CZ-43846 Zatec, Czech Republic Hop Res Inst Co Ltd Zatec Czech Republic CZ-43846 Zatec, Czech Republic
Titolo Testata:
BIOLOGIA PLANTARUM
fascicolo: 1, volume: 44, anno: 2001,
pagine: 7 - 12
SICI:
0006-3134(2001)44:1<7:EIVMAR>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-SUSPENSION CULTURES; TISSUE-CULTURE; HOP; PLANTLETS; INVITRO; PHOTOSYNTHESIS; CULTIVATION; SEEDLINGS; TOBACCO; GROWTH;
Keywords:
gelling agents; glucose; hop; maltose; micropropagation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Lipavska, H Czech Univ Agr, Fac Agron, Lab Tissue Culture, Kamycka 129, CZ-16521 Prague 6, Czech Republic Czech Univ Agr Kamycka 129 Prague Czech Republic 6 h Republic
Citazione:
I. Smykalova et al., "Efficient in vitro micropropagation and regeneration of Humulus lupulus onlow sugar, starch-Gelrite media", BIOL PLANT, 44(1), 2001, pp. 7-12

Abstract

Several Czech and foreign hop mericlones were tested in vitro for efficiency of green callus formation and plant regeneration from internodal or nodal explants. Modified MS media gelled either with agar, starch or a mixture of potato starch and Gelrite, supplemented with different concentrations ofeither glucose or maltose, were investigated. Two mericlones of Czech hop (Osvald 72 no. 5216 and Sladek no. 6908) were studied in more details because of their different regeneration capacities. The HPLC analysis of medium sugar concentrations after the explant cultivation has revealed slow uptakeof sugar from the medium. Presence of glucose at concentration of 45 g dm(-3) in agar medium resulted in a decreased number of nodes compared to the control with 30 g dm(-3) of glucose. The use of a mixture of potato starch plus Gelrite instead of routinely used agar and decreasing the medium glucose concentration to 15 g dm(-3) proved to be most efficient for multiplication rate. The use of this medium results in lower cost of micropropagation of healthy hop cultures without exhibition of vitrification.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 23:50:05