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Titolo:
Assessment of P450 induction in the marmoset monkey using targeted anti-peptide antibodies
Autore:
Schulz, TG; Thiel, R; Neubert, D; Brassil, PJ; Schulz-Utermoehl, T; Boobis, AR; Edwards, RJ;
Indirizzi:
Hammersmith Hosp, Imperial Coll, Sch Med, Div Med,Sect Clin Pharmacol, London W12 0NN, England Hammersmith Hosp London England W12 0NN armacol, London W12 0NN, England Free Univ Berlin, Inst Clin Pharmacol & Toxicol, D-14195 Berlin, Germany Free Univ Berlin Berlin Germany D-14195 Toxicol, D-14195 Berlin, Germany Servier Res & Dev Ltd, Slough SL3 6HH, Berks, England Servier Res & Dev Ltd Slough Berks England SL3 6HH L3 6HH, Berks, England
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
fascicolo: 1, volume: 1546, anno: 2001,
pagine: 143 - 155
SICI:
0167-4838(20010309)1546:1<143:AOPIIT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
PREGNANE-X-RECEPTOR; CALLITHRIX-JACCHUS; LIVER-MICROSOMES; ANTIPEPTIDE ANTIBODIES; HUMAN CYTOCHROME-P450S; HUMAN HEPATOCYTES; RAT-LIVER; ENZYMES; CYP1A2; IDENTIFICATION;
Keywords:
cytochrome P450; cytochrome P450 antibody; anti-peptide antibody; P450 induction; P450 activity; (marmoset monkey);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Edwards, RJ Hammersmith Hosp, Imperial Coll, Sch Med, Div Med,Sect Clin Pharmacol, Du Cane Rd, London W12 0NN, England Hammersmith Hosp Du Cane Rd London England W12 0NN N, England
Citazione:
T.G. Schulz et al., "Assessment of P450 induction in the marmoset monkey using targeted anti-peptide antibodies", BBA-PROT ST, 1546(1), 2001, pp. 143-155

Abstract

The identity and expression of hepatic P450 enzymes in marmosets was investigated using a panel of anti-peptide antibodies originally targeted against human P450 enzymes. In immunoblotting, of 12 antibodies examined, 10 bound specifically to bands in marmoset liver microsomal fraction correspondingto P450 enzymes. It is proposed that these represent marmoset CYP1A1, CYP1A2, CYP2A, CYP2B, CYP2C forms (CYP2C-1 and CYP2C-2), CYP2D19, CYP3A21 and another CYP3A form (CYP3A-m). The antibodies, together with an anti-marmosetCYP2E1 antibody, were used to investigate the expression of 10 P450 enzymes in marmosets treated with P450-inducing chemicals. Treatment with phenobarbitone caused CYP2B, CYP2C-2 and CYP3A21 levels to increase, rifampicin caused increases in CYP2B and CYP2C-1 and a decrease in CYP3A21 levels, whereas dioxin caused CYP1A1 and CYP1A2 levels to increase and CYP2E1 levels to decrease. Clofibric acid did not induce any P450. P450 enzyme activities were assessed using 8 different substrates and increases were found after treatment with phenobarbitone, rifampicin, and dioxin. However, due to speciesdifferences in substrate selectivity, it proved difficult to ascribe thesechanges to individual P450 enzymes. Thus, the use of anti-peptide antibodies provides a more informative way of assessing the levels of specific P450enzymes than enzyme activity measurements. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 06:22:34