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Titolo:
Human triglyceride-rich lipoprotein apo E kinetics and its relationship toLDL apo B-100 metabolism
Autore:
Millar, JS; Lichtenstein, AH; Ordovas, JM; Dolnikowski, CG; Schaefer, EJ;
Indirizzi:
Tufts Univ, Lipid Metab Lab, Jean Mayer Human Nutr Res Ctr Aging, USDA, Boston, MA 02111 USA Tufts Univ Boston MA USA 02111 Res Ctr Aging, USDA, Boston, MA 02111 USA Tufts Univ, Mass Spectrometry Lab, Jean Mayer Human Nutr Res Ctr Aging, USDA, Boston, MA 02111 USA Tufts Univ Boston MA USA 02111 Res Ctr Aging, USDA, Boston, MA 02111 USA
Titolo Testata:
ATHEROSCLEROSIS
fascicolo: 2, volume: 155, anno: 2001,
pagine: 477 - 485
SICI:
0021-9150(200104)155:2<477:HTLAEK>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY LIPOPROTEINS; APOLIPOPROTEIN-E METABOLISM; VERY-LOW-DENSITY; HUMAN-BLOOD-PLASMA; CHYLOMICRON REMNANTS; TRANSGENIC MICE; BINDING; HYPERTRIGLYCERIDEMIA; OVEREXPRESSION; PROTEOGLYCANS;
Keywords:
lipoprotein apo E; kinetics; apo B-100 metabolism;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Schaefer, EJ Tufts Univ, Lipid Metab Lab, Jean Mayer Human Nutr Res Ctr Aging, USDA, 711 Washington St, Boston, MA 02111 USA Tufts Univ 711 Washington St Boston MA USA 02111 MA 02111 USA
Citazione:
J.S. Millar et al., "Human triglyceride-rich lipoprotein apo E kinetics and its relationship toLDL apo B-100 metabolism", ATHEROSCLER, 155(2), 2001, pp. 477-485

Abstract

Apolipoprotein (apo) E is a multifunctional protein that can act as a ligand for lipoprotein receptors. The receptor-mediated clearance of the triglyceride-rich lipoproteins (TRL) chylomicrons and VLDL from plasma is, in part, dependent on apo E. Enrichment of VLDL with apo E is thought to enhance receptor-mediated clearance of VLDL resulting in a low rate of conversion of VLDL to LDL. However. the kinetic mechanism controlling the concentrationof apo E in VLDL is not known. We conducted kinetic studies on apo E in the TRL fraction (d < 1,006 g/ml) and apo B-100 in the TRL acid LDL (d = 1.019-1.063 g/ml) fractions to assess the kinetic determinants of apo E concentration in TRL and to determine the effects that TRL apo E production and clearance rates have on the production rate of LDL apo B-100. Nineteen males between the ages of 24 and 73 underwent a primed-constant infusion with deuterated leucine tracer in the constantly-fed state. Apo B-LOO from TRL and LDL, and apo E from TRL were isolated and their tracer incorporation measured by gas chromatography;mass spectrometry. The residence time and production rates of each protein were determined from the kinetic data using, the SAAM II modeling program. The residence time and production rate of TRL apo E were about one-half that of TRL apo B-100 (1.8 <plus/minus> 1.0 vs. 2.9 +/- 2.1 h and 14.5 +/- 11.0 vs. 27.6 +/- 17.3 mg/kg per day, respectively). The production rate of TRL apo E was weakly correlated with the production rate of TRL apo B-100 (r = 0.324, P = 0.07). Multiple regression analysis showed that the residence time of TRL apo B-100 and the relative TRL apo E production rate (relative to the TRL apo B100 production rate) were negatively associated with LDL apo B-100 production rate, accounting for 68% of itsvariability. We conclude that (1) the concentration of apo E in TRL is highly correlated to its production rate, suggesting that production rate regulates the TRL apo E concentration. and (2) individuals with a relatively short TRL apo B-100 residence time and those producing TRL with a relatively low apo E content have: the highest LDL apo B-100 production rates. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 16:04:09