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Titolo:
A quick method for the assessment of activity and inhibition of fish amylases
Autore:
Fernandez, I; Diaz, M; Martinez, T; Moyano, FJ;
Indirizzi:
Univ Almeria, Dept Biol Aplicada, Escuela Politecn Super, La Canada De SanUrbano, Almeria, Spain Univ Almeria La Canada De San Urbano Almeria Spainrbano, Almeria, Spain
Titolo Testata:
AQUACULTURE NUTRITION
fascicolo: 1, volume: 7, anno: 2001,
pagine: 19 - 23
SICI:
1353-5773(200103)7:1<19:AQMFTA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Keywords:
alpha-amylase; fish; inhibition; starch-agarose gel;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Moyano, FJ Univ Almeria, Dept Biol Aplicada, Escuela Politecn Super, La Canada De SanUrbano, Almeria, Spain Univ Almeria La Canada De San Urbano Almeria Spain ria, Spain
Citazione:
I. Fernandez et al., "A quick method for the assessment of activity and inhibition of fish amylases", AQUAC NUTR, 7(1), 2001, pp. 19-23

Abstract

A sensitive and quick method was developed to determine the presence of alpha -amylase in the gut of aquatic organisms, as well as its sensitivity toinhibitors. The assay is based on the utilization of Petri dishes filled with starch-agarose gel as a substrate for the enzyme solution, which is placed in small wells punched in the surface. Circular zones produced by the action of amylase remain colourless after staining with lugol. Pure commercial porcine amylase was used to fit the better conditions for developing theassay (1 g L-1 starch in the gels, 4 h of incubation). The diameter of thecleared zones were related to the activity of enzyme and the method detected linearly amylase activity in a range of 2-20 U well(-1) so it was used to reveal the presence of amylase in digestive extracts obtained from different sparid fish. The method was also used to evaluate the effect produced by a specific inhibitor on fish amylases, showing a linear response when theratio inhibitor:enzyme (in units) changed from 20:1 to 2:1. Comparison of the cleared zones produced by amylases of sparid fish in the presence or absence of inhibitor, revealed differences in their sensitivity to inhibition, which ranged from 15 to 50% of total activity. The assay is proposed for a preliminary evaluation of possible inhibitors contained in feedstuffs used in fish feeding.

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Documento generato il 15/07/20 alle ore 21:35:43