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Titolo:
In70 of plasmid pAX22, a bla(VIM-1)-containing integron carrying a new aminoglycoside phosphotransferase gene cassette
Autore:
Riccio, ML; Pallecchi, L; Fontana, R; Rossolini, GM;
Indirizzi:
Univ Siena, Dipartimento Biol Mol, Sez Microbiol, I-53100 Siena, Italy Univ Siena Siena Italy I-53100 Mol, Sez Microbiol, I-53100 Siena, Italy Univ Verona, Dipartimento Patol, Sez Microbiol, I-37134 Verona, Italy UnivVerona Verona Italy I-37134 l, Sez Microbiol, I-37134 Verona, Italy
Titolo Testata:
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
fascicolo: 4, volume: 45, anno: 2001,
pagine: 1249 - 1253
SICI:
0066-4804(200104)45:4<1249:IOPPAB>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
METALLO-BETA-LACTAMASE; AERUGINOSA CLINICAL ISOLATE; PSEUDOMONAS-AERUGINOSA; TRIMETHOPRIM-SULFAMETHOXAZOLE; RESISTANCE GENES; RECOMBINATION; SEQUENCE; BLA(VIM); CARRIES; BORNE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Rossolini, GM Univ Siena, Dipartimento Biol Mol, Sez Microbiol, Via Laterina 8, I-53100 Siena, Italy Univ Siena Via Laterina 8 Siena Italy I-53100 Siena, Italy
Citazione:
M.L. Riccio et al., "In70 of plasmid pAX22, a bla(VIM-1)-containing integron carrying a new aminoglycoside phosphotransferase gene cassette", ANTIM AG CH, 45(4), 2001, pp. 1249-1253

Abstract

An Achromobacter xylosoxydans strain showing broad-spectrum resistance to beta -lactams (including carbapenems) and aminoglycosides was isolated at the University Hospital of Verona (Verona, Italy), This strain was found to produce metallo-beta -lactamase activity and to harbor a 30-kb nonconjugative plasmid, named pAX22, carrying a bla(VIM-1) determinant inserted into a class 1 integron, Characterization of this integron, named In70, revealed an original array of four gene cassettes containing, respectively, the bla(vim-1) gene and three different aminoglycoside resistance determinants, including an aacA4 allele, a new aph-like gene named aphA15, and an aadA1 allele, The aphA15 gene is the first example of an aph-like gene carried on a mobile gene cassette, and its product exhibits close similarity to the APH(3')-IIa aminoglycoside phosphotransferase encoded by Tn5 (36% amino acid identity) and to an APH(3')-IIb enzyme from Pseudomonas aeruginosa (38% amino acid identity). Expression of the cloned aphA15 gene in Escherichia coli reduced the susceptibility to kanamycin and neomycin as well as (slightly) to amikacin, netilmicin, and streptomycin, Characterization of the 5' and 3' conserved segments of In70 and of their flanking regions showed that In70 belongs to the group of class 1 integrons associated with defective transposon derivatives originating from Tn402-like elements. The structure of the 3'conserved segment indicates the closest ancestry with members of the In0-In2 lineage. In70, with its array of cassette-borne resistance genes, can mediate broad-spectrum resistance to most beta -lactams and aminoglycosides.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 13:48:35