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Titolo:
Measurement of ionised magnesium in HEPES-buffered serum samples
Autore:
Gow, IF;
Indirizzi:
Hannah Res Inst, Anim Physiol Grp, Ayr KA6 5HL, Scotland Hannah Res Inst Ayr Scotland KA6 5HL Physiol Grp, Ayr KA6 5HL, Scotland
Titolo Testata:
ANALYTICA CHIMICA ACTA
fascicolo: 1, volume: 432, anno: 2001,
pagine: 143 - 149
SICI:
0003-2670(20010322)432:1<143:MOIMIH>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE MYOCARDIAL-INFARCTION; IONIZED MAGNESIUM; CARDIOVASCULAR MORTALITY; SULFATE; INFUSION; CALCIUM; BLOOD; PLASMA; PREECLAMPSIA; ARRHYTHMIAS;
Keywords:
ionised magnesium; pH; serum;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Gow, IF Hannah Res Inst, Anim Physiol Grp, Ayr KA6 5HL, Scotland Hannah Res Inst Ayr Scotland KA6 5HL Grp, Ayr KA6 5HL, Scotland
Citazione:
I.F. Gow, "Measurement of ionised magnesium in HEPES-buffered serum samples", ANALYT CHIM, 432(1), 2001, pp. 143-149

Abstract

The objective of the research is to develop a method of adjusting the pH of serum samples to 7.4, thus allowing measurement of ionised magnesium (Mg2) at physiological pH without the need to maintain samples under anaerobicconditions. Levels of Mg2+ HEPES-buffered samples were compared with results obtained when serum samples were buffered with CO2, and the Mg2+ level at pH 7.4 calculated from the log form of the Siggaard-Andersen equation. The HEPES buffering technique was also used to measure Mg2+ in serum samples from sheep where circulating levels of Mg2+ were elevated by infusion of MgCl2. The mean Mg2+ values obtained when samples (n = 6) were buffered with CO2 or 50 or 100 mM HEPES were not significantly different from each other (mean +/-S.E.M.; 0.675 +/- 0.019, 0.684 +/- 0.015, and 0.669 +/- 0.015, respectively). When MgCl2 was infused into sheep (n = 6), a significant rise in serum Mg2+ was found (mean +/- S.E.M.; basal 0.613 +/- 0.045 mM; peak 1.450 +/- 0.095 mM) when using the HEPES-buffering method for sample pre-treatment (final HEPES concentration was 50 mM). (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 15:41:36