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Titolo:
Acetylcholine increases intracellular Ca2+ in the rat pituitary folliculostellate cells in primary culture
Autore:
Nakajima, Y; Uchiyama, M; Shirai, Y; Sakuma, Y; Kato, M;
Indirizzi:
Nippon Med Sch, Dept Physiol, Tokyo 1138602, Japan Nippon Med Sch Tokyo Japan 1138602 h, Dept Physiol, Tokyo 1138602, Japan
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
fascicolo: 4, volume: 280, anno: 2001,
pagine: E608 - E615
SICI:
0193-1849(200104)280:4<E608:AIICIT>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
HORMONE-SECRETION; STELLATE CELLS; ANTERIOR; RECEPTORS; GLAND; COMMUNICATION; RELEASE; CALCIUM; SYSTEM;
Keywords:
cholinergic modulation; paracrine mechanism; calcium imaging; M-1 receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Kato, M Nippon Med Sch, Dept Physiol, Tokyo 1138602, Japan Nippon Med SchTokyo Japan 1138602 Physiol, Tokyo 1138602, Japan
Citazione:
Y. Nakajima et al., "Acetylcholine increases intracellular Ca2+ in the rat pituitary folliculostellate cells in primary culture", AM J P-ENDO, 280(4), 2001, pp. E608-E615

Abstract

Pituitary folliculostellate cells (FSCs) are thought to partially inhibit pituitary hormone secretion through a paracrine mechanism. In this process,one of the important questions is what factors regulate the function of FSCs. Because ACh is synthesized in and possibly released from the corticotrophs and lactotrophs, we examined whether FSCs respond to ACh by the method of Ca2+ imaging in primary cultured FSCs from male Wistar rats. ACh (30 nM-3 muM) increased intracellular calcium concentration ([Ca2+](i)) of FSCs ina concentration-dependent manner, with an initial rapid rise followed by arelatively sustained increase. The complete block of the response by atropine and pirenzepine suggests involvement of muscarinic receptors. Depletionof the stored Ca2+ by thapsigargin blocked the response completely. Blockers of phospholipase C, U-73122 and neomycin, suppressed significantly the rise of [Ca2+](i). These results suggest that ACh increases [Ca2+](i) in FSCs by activating phospholipase C, presumably through activation of M-1 receptors. The rise in [Ca2+](i) could trigger a variety of Ca2+-dependent cellular processes, including the synthesis and release of bioactive substances,which in turn act on endocrine cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:29:32