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Titolo:
Size matters: Use of YACs, BACs and PACs in transgenic animals
Autore:
Giraldo, P; Montoliu, L;
Indirizzi:
Ctr Nacl Biotecnol, Dept Mol & Cellular Biol, Madrid 28049, Spain Ctr NaclBiotecnol Madrid Spain 28049 Cellular Biol, Madrid 28049, Spain
Titolo Testata:
TRANSGENIC RESEARCH
fascicolo: 2, volume: 10, anno: 2001,
pagine: 83 - 103
SICI:
0962-8819(200104)10:2<83:SMUOYB>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
YEAST ARTIFICIAL CHROMOSOME; BETA-GLOBIN LOCUS; AMYLOID PRECURSOR PROTEIN; GERM-LINE TRANSMISSION; POSITION-INDEPENDENT EXPRESSION; MATRIX-ATTACHMENT REGIONS; NUMBER-RELATED EXPRESSION; HUMAN ALPHA-LACTALBUMIN; MOUSE TYROSINASE GENE; APOLIPOPROTEIN-B GENE;
Keywords:
animal transgenesis; bacterial artificial chromosome; BAC; functional genomics; P1-clone; P1-derived artificial chromosome; PAC; yeast artifical chromosome; YAC;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
189
Recensione:
Indirizzi per estratti:
Indirizzo: Montoliu, L Ctr Nacl Biotecnol, Dept Mol & Cellular Biol, Campus Cantoblanco, Madrid 28049, Spain Ctr Nacl Biotecnol Campus Cantoblanco Madrid Spain28049 pain
Citazione:
P. Giraldo e L. Montoliu, "Size matters: Use of YACs, BACs and PACs in transgenic animals", TRANSGEN RE, 10(2), 2001, pp. 83-103

Abstract

In 1993, several groups, working independently, reported the successful generation of transgenic mice with yeast artificial chromosomes (YACs) using standard techniques. The transfer of these large fragments of cloned genomic DNA correlated with optimal expression levels of the transgenes, irrespective of their location in the host genome. Thereafter, other groups confirmed the advantages of YAC transgenesis and position-independent and copy number-dependent transgene expression were demonstrated in most cases. The transfer of YACs to the germ line of mice has become popular in many transgenic facilities to guarantee faithful expression of transgenes. This techniquewas rapidly exported to livestock and soon transgenic rabbits, pigs and other mammals were produced with YACs. Transgenic animals were also produced with bacterial or P1-derived artificial chromosomes (BACs/PACs) with similar success. The use of YACs, BACs and PACs in transgenesis has allowed the discovery of new genes by complementation of mutations, the identification of key regulatory sequences within genomic loci that are crucial for the proper expression of genes and the design of improved animal models of human genetic diseases. Transgenesis with artificial chromosomes has proven usefulin a variety of biological, medical and biotechnological applications and is considered a major breakthrough in the generation of transgenic animals. In this report, we will review the recent history of YAC/BAC/PAC-transgenic animals indicating their benefits and the potential problems associated with them. In this new era of genomics, the generation and analysis of transgenic animals carrying artificial chromosome-type transgenes will be fundamental to functionally identify and understand the role of new genes, included within large pieces of genomes, by direct complementation of mutations or by observation of their phenotypic consequences.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 19:27:32