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Titolo:
Umbilical cord blood progeny cells that retain a CD34+phenotype after ex vivo expansion have less engraftment potential than unexpended CD34+cells
Autore:
Xu, RL; Reems, JA;
Indirizzi:
Blood Syst Res Lab, Tempe, AZ USA Blood Syst Res Lab Tempe AZ USABlood Syst Res Lab, Tempe, AZ USA
Titolo Testata:
TRANSFUSION
fascicolo: 2, volume: 41, anno: 2001,
pagine: 213 - 218
SICI:
0041-1132(200102)41:2<213:UCBPCT>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM CULTURE; HUMAN STEM-CELLS; HEMATOPOIETIC-CELLS; REPOPULATING CELLS; UNRELATED DONORS; BREAST-CANCER; NOD/SCID MICE; SELF-RENEWAL; IN-VITRO; TRANSPLANTATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Reems, JA NW Tissue Ctr, 921 Terry Ave, Seattle, WA 98104 USA NW Tissue Ctr 921 Terry Ave Seattle WA USA 98104 e, WA 98104 USA
Citazione:
R.L. Xu e J.A. Reems, "Umbilical cord blood progeny cells that retain a CD34+phenotype after ex vivo expansion have less engraftment potential than unexpended CD34+cells", TRANSFUSION, 41(2), 2001, pp. 213-218

Abstract

BACKGROUND: Because of the limitation of cell numbers associated with cordblood harvests, there is a need to determine the efficacy of using ex vivo-expanded cord blood cells in a transplantation setting. in this study, limiting-dilution analysis was used in nonobese diabetic mice with severe combined immunodeficiency (NOD/SCID) to compare the engraftment potential of progeny cells expressing the CD34+ phenotype after expansion with that of uncultured CD34+ cells. STUDY DESIGN AND METHODS: Cord blood CD34+ cells were cultured in Iscove'smodified Dulbecco medium supplemented with 10-percent fetal calf serum (FCS) and IL-6, SCF, megakaryocyte growth and development factor, and Flt3 ligand. The resulting ex vivo-expanded products were assessed for total numbers of nucleated cells, CD34+ cells, and CFUs and long-term culture-initiating cell activity. The engraftment potentials of cultured progeny CD34+ cellsand uncultured CD34+ cells were determined by using NOD/SCID mice. RESULTS: After 14 days of culture, total nucleated cell counts increased over input values by 180 +/- 59-fold, CD34+ cell numbers by 44 +/- 13-fold, CFU activity by 23 +/- 5-fold, and long-term culture-initiating cell activity by 20 +/- 6-fold (mean +/- SD; n = 6). The frequency of SCID-repopulating cells (SRC) in mice transplanted with uncultured products was 1 per 20,000 CD34+ cells (95% CI, 1:10,000-1:38,000) and that in mice receiving ex vivo-expanded products was 1 per 418,000 progeny CD34+ cells (95% CI, 1:158,000-1:1,1 00,000). Taken together, these data indicated that, after 2 weeks of culture, there was a modest twofold increase in the total number of SRCs. However, the levels of human CD45 cell engraftment in NOD/SCID recipients of progeny CD34+ cells were significantly tower than those in mice receiving equivalent numbers of uncultured CD34+ cells (p<0.05). CONCLUSION: Umbilical cord blood progeny cells retaining a CD34+ phenotypeafter ex vivo expansion have less engraftment potential than do unexpandedCD34+ cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 23:08:55