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Titolo:
In vitro double fertilization in Nicotiana tabacum (L.): the role of cell volume in cell fusion
Autore:
Sun, MX; Moscatelli, A; Yang, HY; Cresti, M;
Indirizzi:
Univ Siena, Dept Environm Sci, I-53100 Siena, Italy Univ Siena Siena Italy I-53100 , Dept Environm Sci, I-53100 Siena, Italy Wuhan Univ, Key Lab MOE Dev Biol, Wuhan 430072, Peoples R China Wuhan Univ Wuhan Peoples R China 430072 l, Wuhan 430072, Peoples R China Univ Messina, Inst Bot, I-98165 Messina, Italy Univ Messina Messina Italy I-98165 ina, Inst Bot, I-98165 Messina, Italy
Titolo Testata:
SEXUAL PLANT REPRODUCTION
fascicolo: 4, volume: 13, anno: 2001,
pagine: 225 - 229
SICI:
0934-0882(200102)13:4<225:IVDFIN>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO; INVITRO FERTILIZATION; UNILAMELLAR VESICLES; EGG CELLS; MAIZE; ELECTROFUSION; PROTOPLASTS; CURVATURE; SINGLE; PLANTS;
Keywords:
Nicotiana tabacum; in vitro fertilization; cell fusion; cell volume; membrane reorganization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Cresti, M Univ Siena, Dept Environm Sci, Via Laterina 8, I-53100 Siena, Italy Univ Siena Via Laterina 8 Siena Italy I-53100 3100 Siena, Italy
Citazione:
M.X. Sun et al., "In vitro double fertilization in Nicotiana tabacum (L.): the role of cell volume in cell fusion", SEX PLANT R, 13(4), 2001, pp. 225-229

Abstract

The role of cell volume in regulating cell fusion was investigated by in vitro fertilization to elucidate mechanisms involved in double fertilization. The results revealed that in our model gamete fusion was more efficient than the fusion of somatic cells under the same conditions. The fusion of selected mesophyll protoplasts of different sizes and their fusion with chloroplasts demonstrated that cell volume ratio played a role in this process: as the ratio increased, fusion was more efficient and faster. When one of the cells was as small as a sperm, the formation of a round fusion product was faster. This might explain why gamete fusion was highly efficient in allin vitro germ cell fusion systems. This finding may also explain why spermevolved as small cells. The results reported here will be useful for interpreting and evaluating data of in vitro fertilization experiments and for distinguishing gamete-specific characters.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 18:44:51