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Titolo:
Compositional and functional changes of pulmonary surfactant in a guinea-pig model of chronic asthma
Autore:
Cheng, G; Ueda, T; Sugiyama, K; Toda, M; Fukuda, T;
Indirizzi:
Dokkyo Univ, Sch Med, Dept Pulm Med & Clin Immunol, Mibu, Tochigi 3210293,Japan Dokkyo Univ Mibu Tochigi Japan 3210293 munol, Mibu, Tochigi 3210293,Japan
Titolo Testata:
RESPIRATORY MEDICINE
fascicolo: 3, volume: 95, anno: 2001,
pagine: 180 - 186
SICI:
0954-6111(200103)95:3<180:CAFCOP>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE LUNG INJURY; IN-VITRO CONVERSION; SMALL AIRWAYS; EXOGENOUS SURFACTANT; ALVEOLAR SURFACTANT; VIVO FUNCTION; RABBIT LUNG; PROTEIN-A; METABOLISM; SUBTYPES;
Keywords:
alveolar inflammation; surface tension; surfactant subtype conversion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Cheng, G Dokkyo Univ, Sch Med, Dept Pulm Med & Clin Immunol, Mibu, Tochigi3210293,Japan Dokkyo Univ Mibu Tochigi Japan 3210293 bu, Tochigi 3210293,Japan
Citazione:
G. Cheng et al., "Compositional and functional changes of pulmonary surfactant in a guinea-pig model of chronic asthma", RESP MED, 95(3), 2001, pp. 180-186

Abstract

Recent studies have found that severe surfactant dysfunction occurs duringan asthma attack, but the changes in surfactant in a guinea-pig model of chronic asthma have not been studied. We therefore analysed the surfactant recovered from guinea-pigs after repeated inhalation of ovalbumin to see if the surfactant recovered from chronic asthmatic lungs would be intrinsically altered. Guinea pigs immunized through repeated inhalation of aerosolizedovalbumin (OA) were exposed to the antigen once a week for a month. Twenty-four hours after the last challenge the alveolar wash was recovered. We calculated saturated phosphatidylcholine (Sat-PC) and total protein (TP) poolsizes in alveolar spaces. Surfactant subtype conversion of large aggregatesurfactant (LA) to small aggregate surfactant was studied in vitro by means of the surface area cycling technique. The phospholipid composition of LAwas analysed by thin layer chromatography and the surface activity of LA was also determined. We found decreased surfactant pool sizes, decreased ratio of Sat-PC to TP in alveolar lavages in asthma groups, and surface activity of the surfactant recovered from asthmatic lungs to be inferior to that of the controls. Accelerated surfactant subtype conversion in vitro was also noted in the lungs of asthmatic animal models. In addition, the changes in phospholipid compositions which were similar to the pattern of acute lunginjury suggested that alveolar inflammation might be involved in the pathogenesis of chronic asthma. These results indicate that surfactant is intrinsically abnormal in chronically asthmatic lungs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 19:40:19