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Titolo:
Isolation and characterization of a polygalacturonase-inhibiting protein from potato leaves. Accumulation in response to salicylic acid, wounding andinfection
Autore:
Machinandiarena, MF; Olivieri, FP; Daleo, GR; Oliva, CR;
Indirizzi:
Univ Mar del Plata, Fac Ciencias Exactas & Nat, Inst Invest Biol, RA-7600 Mar Del Plata, Argentina Univ Mar del Plata Mar Del Plata Argentina RA-7600 Del Plata, Argentina
Titolo Testata:
PLANT PHYSIOLOGY AND BIOCHEMISTRY
fascicolo: 2, volume: 39, anno: 2001,
pagine: 129 - 136
SICI:
0981-9428(200102)39:2<129:IACOAP>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHASEOLUS-VULGARIS L; HOST-PATHOGEN INTERACTIONS; COLLETOTRICHUM-LINDEMUTHIANUM; FUNGAL POLYGALACTURONASES; ISOGENIC LINES; PLANT DEFENSE; PURIFICATION; ELICITOR; ELEMENTS; ENDOPOLYGALACTURONASE;
Keywords:
Phytophthora infestans; plant defence; polygalacturonase-inhibiting protein; Solanum tuberosum;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Oliva, CR Univ Mar del Plata, Fac Ciencias Exactas & Nat, Inst Invest Biol, CC 1245,RA-7600 Mar Del Plata, Argentina Univ Mar del Plata CC 1245 Mar Del Plata Argentina RA-7600 tina
Citazione:
M.F. Machinandiarena et al., "Isolation and characterization of a polygalacturonase-inhibiting protein from potato leaves. Accumulation in response to salicylic acid, wounding andinfection", PL PHYS BIO, 39(2), 2001, pp. 129-136

Abstract

A polygalacturonase-inhibiting protein (PGIP) was purified from potato leaves (Solanum tuberosum L. cv. Spunta). The protein was cell wall bound and had a molecular ma ss of 41 kDa, as determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and gel filtration. Potato PGIP showed a broad inhibitory activity against crude polygalacturonase preparations fromseveral fungi grown in liquid culture. Using heterologous antibodies in western blot experiments, it was shown that the levels of PGIP are increased in potato leaves in response to wounding, treatment with salicylic acid andinfection with Phytophthora infestans during an incompatible interaction. The data support the hypothesis that, synthesis of PGIP may constitute an active defence mechanism of potato plants that is elicited by signal molecules known to induce plant defence genes. (C) 2001 Editions scientifiques et medicales Elsevier SAS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 20:06:24