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Titolo:
Molecular mapping of a new gene for resistance to frogeye leaf spot of soya bean in 'Peking'
Autore:
Yang, W; Weaver, DB; Nielsen, BL; Qiu, J;
Indirizzi:
Auburn Univ, Dept Agron & Soils, Auburn, AL 36849 USA Auburn Univ Auburn AL USA 36849 Dept Agron & Soils, Auburn, AL 36849 USA Auburn Univ, Alabama Agr Expt Stn, Auburn, AL 36849 USA Auburn Univ Auburn AL USA 36849 labama Agr Expt Stn, Auburn, AL 36849 USA Auburn Univ, Dept Biol Sci, Auburn, AL 36849 USA Auburn Univ Auburn AL USA 36849 Univ, Dept Biol Sci, Auburn, AL 36849 USA LI COR Inc, Div Biotechnol, Lincoln, NE 68504 USA LI COR Inc Lincoln NE USA 68504 nc, Div Biotechnol, Lincoln, NE 68504 USA
Titolo Testata:
PLANT BREEDING
fascicolo: 1, volume: 120, anno: 2001,
pagine: 73 - 78
SICI:
0179-9541(200102)120:1<73:MMOANG>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOYBEAN CYST-NEMATODE; DNA MARKERS; DISEASE RESISTANCE; AFLP MARKERS; RFLP MARKERS; LINKAGE MAP; INTEGRATION; GENOME; POLYMORPHISM; POPULATION;
Keywords:
Glycine max; frogeye leaf spot; resistance; Rcs(Peking) gene; molecular mapping; AFLP; SSR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Weaver, DB Auburn Univ, Dept Agron & Soils, Auburn, AL 36849 USA Auburn Univ Auburn AL USA 36849 & Soils, Auburn, AL 36849 USA
Citazione:
W. Yang et al., "Molecular mapping of a new gene for resistance to frogeye leaf spot of soya bean in 'Peking'", PLANT BREED, 120(1), 2001, pp. 73-78

Abstract

Amplified fragment length polymorphism (AFLP) and microsatellite (simple sequence repeat, SSR) techniques were used to map the Rcs(Peking) gene, which is resistant to most isolates of Cercospora sojina in the soya bran cultivar 'Peking'. The mapping was conducted using a defined F-2 population derived from the cross of 'Peking' (resistant) x 'Lee' (susceptible). Of 64 EcoRI and MseI primer combinations, 30 produced polymorphisms between the two parents. The F-2 population, consisting of 116 individuals, was screened with the 30 AFLP primer pairs and three mapped SSR markers to detect markers possibly linked to Rcs(Peking). One AFLP marker amplified by primer pair E-AAC/M-CTA and one SSR marker Satt244 were identified to be linked to Rcs(Peking). The gene was located within a 2.1-cM interval between markers AACCTA178 and Satt244, 1.1 cM from Satt244 and 1.0 cM from AACCTA178. Since the SSR markers Satt244 and Satt431 have been mapped to molecular linkage group (LC) J of soya bean, the Rcs(Peking) resistance gene was putatively locatedon the LG J. This will provide soya bean breeders an opportunity to use these markers For marker-assisted selection for frogeye leaf spot resistance in soya bean.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 00:39:02