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Titolo:
Characteristics of choline transport across the blood-brain barrier in mice: Correlation with in vitro data
Autore:
Murakami, H; Sawada, N; Koyabu, N; Ohtani, H; Sawada, Y;
Indirizzi:
Kyushu Univ, Grad Sch Pharmaceut Sci, Dept Medicopharmaceut Sci, Higashi Ku, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 ci, Higashi Ku, Fukuoka 8128582, Japan
Titolo Testata:
PHARMACEUTICAL RESEARCH
fascicolo: 12, volume: 17, anno: 2000,
pagine: 1526 - 1530
SICI:
0724-8741(200012)17:12<1526:COCTAT>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
ORGANIC CATION TRANSPORTER; CAPILLARY ENDOTHELIAL-CELLS; FUNCTIONAL-CHARACTERIZATION; RAT-BRAIN; CLONING; EXPRESSION; NEURONS; METABOLISM; ROCT1; OCTN1;
Keywords:
choline; in situ brain perfusion technique; bloodbrain barrier; organic cation transporter; in vivo and in vitro correlation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Sawada, Y Kyushu Univ, Grad Sch Pharmaceut Sci, Dept Medicopharmaceut Sci,Higashi Ku, 3-1-1 Maidashi, Fukuoka 8128582, Japan Kyushu Univ 3-1-1 Maidashi Fukuoka Japan 8128582 8128582, Japan
Citazione:
H. Murakami et al., "Characteristics of choline transport across the blood-brain barrier in mice: Correlation with in vitro data", PHARM RES, 17(12), 2000, pp. 1526-1530

Abstract

Purpose. We examined the functional properties of choline transport acrossthe blood-brain barrier (BBB) in mice. We compared the kinetic parameters and transport properties with those found in our in vitro uptake experiments using mouse brain capillary endothelial cells (MBEC4). Methods, The permeability coefficient-surface area product (PS) values of [H-3]choline at the BBB were estimated by means of an in situ brain perfusion technique in mice. Results. [H-3]Choline uptake was well described by a two-component model: a saturable component and a nonsaturable linear component. The [H-3]cholineuptake was independent of pH and Na+, but was significantly decreased by the replacement of Na+ with K+. Various basic drugs, including substrates and inhibitors of the organic cation transporter, significantly inhibited the[H-3]choline uptake. These in situ (in vivo) results corresponded well to the in vitro results and suggest that the choline transporter at the BBB isa member of the organic cation transporter (OCT) family. Conclusion. The choline transport mechanism at the BBB is retained in MBEC4.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 23:33:49