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Titolo:
Overexpression of active TGF-beta-1 in the murine knee joint: evidence forsynovial-layer-dependent chondro-osteophyte formation
Autore:
Bakker, AC; van de Loo, FAJ; van Beuningen, HM; Sime, P; van Lent, PLEM; van der Kraan, PM; Richards, CD; van den Berg, WB;
Indirizzi:
Univ Nijmegen Hosp, Dept Rheumatol, NL-6525 GA Nijmegen, Netherlands Univ Nijmegen Hosp Nijmegen Netherlands NL-6525 GA Nijmegen, Netherlands McMaster Univ, Dept Pathol, Hamilton, ON L8N 3Z5, Canada McMaster Univ Hamilton ON Canada L8N 3Z5 ol, Hamilton, ON L8N 3Z5, Canada
Titolo Testata:
OSTEOARTHRITIS AND CARTILAGE
fascicolo: 2, volume: 9, anno: 2001,
pagine: 128 - 136
SICI:
1063-4584(200102)9:2<128:OOATIT>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; MEDIATED GENE-TRANSFER; COLLAGEN-INDUCED ARTHRITIS; CARTILAGE PANNUS JUNCTION; ZYMOSAN-INDUCED ARTHRITIS; NECROSIS-FACTOR-ALPHA; IN-VIVO; RHEUMATOID-ARTHRITIS; LINING CELLS; TRANSFORMING GROWTH-FACTOR-BETA-1;
Keywords:
TGF-beta; chondro-osteophyte formation; cartilage; adenoviral vector;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Bakker, AC Univ Nijmegen Hosp, Dept Rheumatol, NL-6525 GA Nijmegen, Netherlands Univ Nijmegen Hosp Nijmegen Netherlands NL-6525 GA etherlands
Citazione:
A.C. Bakker et al., "Overexpression of active TGF-beta-1 in the murine knee joint: evidence forsynovial-layer-dependent chondro-osteophyte formation", OSTEO CART, 9(2), 2001, pp. 128-136

Abstract

Objective: To investigate the impact of a prolonged and constant active TGF-beta expression by the synovial lining cells on cartilage and ligamentousjoint structures in vivo. Design: An adenoviral vector (AdTGF-beta1(223.225)) was used for the overexpression of active TGF-beta1 in knee joints of C57B1/6 mice. Results: It was found that physiological relevant levels of active TGF-beta1 produced by the synovial lining layer resulted in histopathological changes: hyperplasia of synovium and chondro-osteophyte formation at the so-called chondro-synovial junctions. No histological changes were seen after intra-articular injection of an empty control vector (AdDL70-3) or by overexpression of latent TGF-beta1 (AdTGF-beta1). The predominant site of TGF-beta production in osteoarthritis (OA) and rheumatoid arthritis (RA) is the synovial lining layer. To address the question whether the TGF-beta -induced changes were related to the expression site in the synovial lining, the synovial lining layer was depleted by local treatment with liposomes encapsulating clodronate. Depletion of the lining resulted in a dramatic change of TGF-beta1-induced pathology: markedly reduced chondro-osteophyte formation andincreased accumulation of extracellular matrix in the synovium. Conclusion: This study shows that overexpression of active TGF-beta1 in the knee joint results in OA-like changes and suggests the synovial lining cells contribute to the chondro-osteophyte formation. (C) 2001 OsteoArthritisResearch Society International.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 16:05:02