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Titolo:
Analysis of mutational effects at the HPRT locus in human G(0) phase lymphocytes irradiated in vitro with gamma rays
Autore:
Mognato, M; Ferraro, P; Canova, S; Sordi, G; Russo, A; Cherubini, R; Celotti, L;
Indirizzi:
Univ Padua, Dipartimento Biol, I-35121 Padua, Italy Univ Padua Padua Italy I-35121 , Dipartimento Biol, I-35121 Padua, Italy Univ Insubria, Dipartimento Biol Strutturale & Funz, Varese, Italy Univ Insubria Varese Italy mento Biol Strutturale & Funz, Varese, Italy Ist Nazl Fis Nucl, Lab Nazl Legnaro, Padua, Italy Ist Nazl Fis Nucl Padua Italy Fis Nucl, Lab Nazl Legnaro, Padua, Italy
Titolo Testata:
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
fascicolo: 1-2, volume: 474, anno: 2001,
pagine: 147 - 158
SICI:
1386-1964(20010301)474:1-2<147:AOMEAT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; HUMAN T-LYMPHOCYTES; CHINESE-HAMSTER CELLS; LOW-ENERGY PROTONS; DELETION MUTATIONS; CHROMOSOMAL INSTABILITY; MOLECULAR ANALYSIS; SEQUENCE-ANALYSIS; MUTANT FREQUENCY; MULTIPLEX-PCR;
Keywords:
gamma rays; HPRT mutants; human PBL; deletion mutations;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Celotti, L Univ Padua, Dipartimento Biol, Via U Bassi 58B, I-35121 Padua, Italy Univ Padua Via U Bassi 58B Padua Italy I-35121 21 Padua, Italy
Citazione:
M. Mognato et al., "Analysis of mutational effects at the HPRT locus in human G(0) phase lymphocytes irradiated in vitro with gamma rays", MUT RES-F M, 474(1-2), 2001, pp. 147-158

Abstract

The mutational effects of ionising radiation at the hypoxanthine-guanine phosphoribosyl transferase (HPRT) locus were studied in human peripheral blood G(0) phase lymphocytes irradiated in vitro with gamma rays. The presenceof radiation induced mutants was assessed by selecting the HPRT mutants every week on the basis of 6-thioguanine resistance up to 1 month after irradiation. A dose-related increase of 14.25 x 10(-6) mutants/Gy was measured after an expression time of 7 days. After 2 weeks from culture starting the fraction of clonable cells in irradiated and control cell populations decreased, limiting the measurements of mutant frequency. The mutational spectrum of the HPRT gene was determined by PCR analyses in a total of 99 mutant clones derived from irradiated lymphocytes. The independent origin of mutantclones carrying the same mutation was assessed by analysing the TCR gamma gene rearrangements. The results showed a dose-related increase of deletionmutants up to 3 Gy, whereas point mutation frequency increased only up to 2 Gy. Two preferentially deleted regions were identified; one involving theHPRT exon 3, and another one the 3'-terminal and the 3'-flanking region ofthe gene. One complex mutation involving a non-contiguous deletion of exons 2-5 and 7/8 was observed among the mutants isolated after 3 Gy irradiation. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 01/04/20 alle ore 23:00:03