Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Identification of a nuclear protein ArpN as a component of human SWI/SNF complex and its selective association with a subset of active genes
Autore:
Sung, YH; Choi, EY; Kwon, H;
Indirizzi:
Kyung Hee Univ, Grad Sch EW Med Sci, Dept Neurosci, Seoul 130701, South Korea Kyung Hee Univ Seoul South Korea 130701 rosci, Seoul 130701, South Korea Dankook Univ, Dept Mol Biol, Seoul 140714, South Korea Dankook Univ Seoul South Korea 140714 ol Biol, Seoul 140714, South Korea
Titolo Testata:
MOLECULES AND CELLS
fascicolo: 1, volume: 11, anno: 2001,
pagine: 75 - 81
SICI:
1016-8478(20010228)11:1<75:IOANPA>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
SWI-SNF COMPLEX; GLUCOCORTICOID RECEPTOR; TRANSCRIPTIONAL ACTIVATION; NUCLEOSOME DISRUPTION; HUMAN HOMOLOG; CHROMATIN; BINDING; YEAST; LOCALIZATION; INTERACTS;
Keywords:
beta-actin; ArpN; chromatin immunoprecipitation; chromatin remodeling; hSWI/SNF complex;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Kwon, H Kyung Hee Univ, Grad Sch EW Med Sci, Dept Neurosci, Seoul 130701, South Korea Kyung Hee Univ Seoul South Korea 130701 eoul 130701, South Korea
Citazione:
Y.H. Sung et al., "Identification of a nuclear protein ArpN as a component of human SWI/SNF complex and its selective association with a subset of active genes", MOL CELLS, 11(1), 2001, pp. 75-81

Abstract

The hSWI/SNF complex remodels the chromatin structure to modulate gene expression. The hSWI/SNF complex is a multiprotein complex with at least 10 different proteins in mammals, Tn this study, we identified the 45 kDa subunit of the hSWI/SNF complex as ArpN, an actin-related protein. ArpN has a 36%identity and 50% similarity with the human beta -actin, but cannot be classified into any known class of actin-related proteins. ArpN is exclusively localized within the nucleus and appears as the unbound, chromatin-associated, or nuclear matrix associated forms in the nucleus. In the chromatin immunoprecipitation (ChIP) assay, we found the associations of ArpN with the Ets-2 and c-mycP2 promoter regions in HeLa cells. The promoter regions of the hsp70, cyclophilin, P-globin, TdT, and cd4 genes, however, were not associated with ArpN. The Ets-2 and c-mycP2 genes are expressed actively in HeLacells, but beta -globin, TdT, and cd4 genes are inactive, The hsp70 and cyclophilin genes have a feature of stress-inducibility. These selective associations of ArpN with a subset of active genes support the proposition thatthe requirement of hSWI/SNF complex in gene activation is gene specific.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 09:26:34