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Titolo:
Molecular cloning and cultivar specific expression of MAP kinases from Capsicum annuum
Autore:
Shin, HJ; Lee, DE; Shin, DH; Kim, KU; Kim, HY; Ohashi, Y; Han, O; Baik, MG; Back, K;
Indirizzi:
Chonnam Natl Univ, Biotechnol Res Inst, Dept Genet Engn, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea Kyungpook Natl Univ, Coll Agr, Dept Agron, Taegu 702701, South Korea Kyungpook Natl Univ Taegu South Korea 702701 , Taegu 702701, South Korea Natl Inst Agrobiol Resources, Dept Mol Genet, Tsukuba, Ibaraki 3058602, Japan Natl Inst Agrobiol Resources Tsukuba Ibaraki Japan 3058602 3058602, Japan
Titolo Testata:
MOLECULES AND CELLS
fascicolo: 1, volume: 11, anno: 2001,
pagine: 48 - 54
SICI:
1016-8478(20010228)11:1<48:MCACSE>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED PROTEIN-KINASE; SIGNAL-TRANSDUCTION PATHWAYS; TRANSIENT ACTIVATION; SESQUITERPENE CYCLASE; VIRUS-INFECTION; PLANT DEFENSE; TOBACCO; RESISTANCE; LEAVES; CELLS;
Keywords:
Capsicum annuum; Escherichia coli expression; MAP kinase; ultraviolet C; wounding stress;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Back, K Chonnam Natl Univ, Biotechnol Res Inst, Dept Genet Engn, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 500757, South Korea
Citazione:
H.J. Shin et al., "Molecular cloning and cultivar specific expression of MAP kinases from Capsicum annuum", MOL CELLS, 11(1), 2001, pp. 48-54

Abstract

Two MAP kinases, MK1 and MK2, were cloned from Capsicum annuum (pepper) cv, Subicho using a parsley MAP kinase gene as a heterologous probe. MK1 and MK2 encode stress-inducible protein kinases that can contribute to the response to wounding, UV-C, and cold, MK1 has a 92% amino acid identity with WIPK of tobacco. It was transcriptionally induced in response to wounding. Incontrast, no detectable MK1 transcript was found in unwounded leaves of pepper. MK2 has a high level of amino acid homology to MAP kinases, such as NTF4 and SIPK and nas constitutively expressed in all tissues. Both MK transcripts were downregulated by W-C treatment. Each MK protein activation was independently wound-inducible in a cultivar dependent manner. MK1 is phosphorylated in cv, Pungchon but not cv, Subicho; whereas, the MK2 protein activation by wounding is restricted to cv, Subicho, In addition, de novo synthesis of the MK1 protein and tyrosine phosphorylation was rapidly and transiently induced in cv, Pungchon by wounding, In contrast, it is highly unlikely that the MK1;I protein is produced in cv, Subicho, even though there is an abundant expression of MK1 mRNA after wounding in this cultivar, In Escherichia coli, which overexpresses MK1, autophosphorylation is observed at conserved threonine and tyrosine phosphorylation sites.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 02:09:21