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Titolo:
The use of hyperoxia to induce chronic mild oxidative stress in RPE cells in vitro
Autore:
Honda, S; Hjelmeland, LM; Handa, JT;
Indirizzi:
Univ Calif Davis, Sch Med, Vitreoretinal Res Lab, Dept Ophthalmol, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 Dept Ophthalmol, Davis, CA 95616 USA Univ Calif Davis, Dept Mol & Cellular Biol, Davis, CA 95616 USA Univ CalifDavis Davis CA USA 95616 & Cellular Biol, Davis, CA 95616 USA
Titolo Testata:
MOLECULAR VISION
fascicolo: 10, volume: 7, anno: 2001,
pagine: 63 - 70
SICI:
1090-0535(20010314)7:10<63:TUOHTI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
PIGMENT EPITHELIAL-CELLS; FIBROBLAST GROWTH-FACTOR; IN-VITRO; HEME OXYGENASE; REPLICATIVE SENESCENCE; NUCLEOTIDE-SEQUENCE; GENE-EXPRESSION; ASCORBIC-ACID; LIFE-SPAN; VITAMIN-C;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Honda, S Univ Calif Davis, Sch Med, Vitreoretinal Res Lab, Dept Ophthalmol, 1 Shields Ave, Davis, CA 95616 USA Univ Calif Davis 1 Shields Ave Davis CA USA 95616 s, CA 95616 USA
Citazione:
S. Honda et al., "The use of hyperoxia to induce chronic mild oxidative stress in RPE cells in vitro", MOL VIS, 7(10), 2001, pp. 63-70

Abstract

Purpose: To establish a model of mild and chronic oxidative stress using hyperoxia for retinal pigment epithelial (RPE) cells in vitro. Methods: RPE340 cells and WI38 lung fibroblasts were grown in normal oxygen (20% O-2) and hyperoxia (40% O-2 or 60% O-2). After cell viability was examined, the levels of reactive oxygen intermediates (ROI) by flow cytometryand heme oxygenase-1 (HO-1) mRNA by northern analysis were measured as markers of oxidative stress in both cell types. Proliferative ability and geneexpression pattern of growth factors were studied to demonstrate the phenotypic changes induced by mild oxidative stress upon these cells. Results: While decreased by 60% O-2, 40% O-2 did not affect viability in both cell types, ROI production and HO-1 mRNA expression were elevated in hyperoxia compared to controls, but were inhibited with the antioxidant dehydro-ascorbic acid (DHA). The proliferation of cells by hyperoxia was inhibited in both cell types. The expression of growth factors induced by hyperoxia was cell type dependent. Fibroblast growth factor-2 mRNA was unchanged inRPE cells, but was increased in fibroblasts. Transforming growth factor-beta2 was decreased in RPE cells, but unchanged in fibroblasts. Vascular endothelial growth factor was downregulated in RPE cells, while upregulated in fibroblasts. Connective tissue growth factor was decreased in RPE cells, but was unchanged in fibroblasts. Conclusions: The results demonstrate that hyperoxia induces mild oxidativestress which alters the phenotype of cells in a cell type specific manner.

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Documento generato il 29/09/20 alle ore 00:36:05