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Titolo:
Retinal uptake of intravitreally injected Hsc/Hsp70 and its effect on susceptibility to light damage
Autore:
Yu, Q; Kent, C; Tytell, M;
Indirizzi:
Wake Forest Univ, Bowman Gray Sch Med, Dept Neurobiol & Anat, Winston Salem, NC 27109 USA Wake Forest Univ Winston Salem NC USA 27109 , Winston Salem, NC 27109 USA
Titolo Testata:
MOLECULAR VISION
fascicolo: 8, volume: 7, anno: 2001,
pagine: 48 - 56
SICI:
1090-0535(20010307)7:8<48:RUOIIH>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK PROTEINS; FIBROBLAST GROWTH-FACTOR; STRESS PROTEINS; RAT RETINA; INDUCED APOPTOSIS; COGNATE PROTEIN; HEAT-SHOCK-PROTEIN-70; CHAPERONES; HSP70; CELL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Tytell, M Wake Forest Univ, Bowman Gray Sch Med, Dept Neurobiol & Anat, Winston Salem, NC 27109 USA Wake Forest Univ Winston Salem NC USA 27109 Salem, NC 27109 USA
Citazione:
Q. Yu et al., "Retinal uptake of intravitreally injected Hsc/Hsp70 and its effect on susceptibility to light damage", MOL VIS, 7(8), 2001, pp. 48-56

Abstract

Purpose: To evaluate the uptake by the rat retina of an intravitreally injected mixture of the constitutive and inducible forms of the 70 kD heat shock protein (Hsc/Hsp70) and test its potential to protect photoreceptors from light damage. Methods: Hsc/Hsp70 and actin (control protein) were labeled with fluorescein (referred to as fl-Hsc/Hsp70 and fl-actin). The labeled proteins were microinjected intravitreally into the normal or light damaged rat eye and each eye collected at three intervals after the injections. Retinal uptake of Hsc/Hsp70 or actin was studied in frozen sections using epifluorescence microscopy and in western blots of retinal homogenates using an anti-fluorescein antibody. Additionally, the cytoprotective effects of Hsc/Hsp70 were tested in rats that first were exposed to bright light (170 ft-c) for 24 h andthen given an intravitreal injection of the protein immediately thereafter. Ten days later, photoreceptor damage was evaluated by measuring the area of the outer nuclear layer at fixed locations along the circumference of the retina. Results: The fluorescein-labeled proteins were detected in the retina one h after administration and were retained there for more than 6 h. They werediffusely distributed, primarily in the nerve fiber layer, ganglion cell layer, and plexiform layers. Fl-Hsc/Hsp70 was also found in the outer nuclear layer (ONL) at 6 h after injection. At 24 h post-injection, the proteins were undetectable by epifluorescence microscopy of retinal sections, but could still be detected in western blots of retinal homogenates. The pattern of protein uptake was similar in light-damaged retinas. Ten days after light damage, the retinas in those eyes that received injections of Hsc/Hsp70 had greater ONL areas compared to either the light-damaged retinas of uninjected eyes or those that had received actin. The difference was statistically significant (p <0.05). Conclusions: Intravitreally injected Hsc/Hsp70 is taken up by retinal cells and, when administered after an acute injury like light damage, increasedthe number of surviving photoreceptors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 16:15:49