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Titolo:
Cloning and characterization of human haspin gene encoding haploid germ cell-specific nuclear protein kinase
Autore:
Tanaka, H; Iguchi, N; Nakamura, Y; Kohroki, J; de Carvalho, CE; Nishimune, Y;
Indirizzi:
Osaka Univ, Microbial Dis Res Inst, Dept Sci Lab Anim Experimentat, Suita,Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 rimentat, Suita,Osaka 5650871, Japan
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 3, volume: 7, anno: 2001,
pagine: 211 - 218
SICI:
1360-9947(200103)7:3<211:CACOHH>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUBUNIT; PHOSPHORYLATION; DIFFERENTIATION; IDENTIFICATION; SEQUENCE; HOMOLOG; MUTANT; CYCLE;
Keywords:
cDNA; haspin; kinase; nucleus; spermiogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Nishimune, Y Osaka Univ, Microbial Dis Res Inst, Dept Sci Lab Anim Experimentat, 3-1 Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ 3-1 Yamadaoka Suita Osaka Japan 5650871 871, Japan
Citazione:
H. Tanaka et al., "Cloning and characterization of human haspin gene encoding haploid germ cell-specific nuclear protein kinase", MOL HUM REP, 7(3), 2001, pp. 211-218

Abstract

We report here the molecular cloning and characterization of human haspin cDNA and its genomic DNA construct. The haspin protein is a unique protein kinase, first isolated from mouse testis, Specifically expressed in mouse testicular germ cells, haspin is suggested to play a role in cell cycle arrest in haploid spermatids, Detection of human haspin by Northern blot analysis showed that the major transcript was 2.8 kilobases long and detected exclusively in the testis, The entire coding region of the human cDNA showed 68% identity with mouse haspin, The predicted amino acid sequence showed strong conservation of the kinase catalytic domain, leucine zipper, potential phosphorylation sites, and MEF2B homologous region, but a relatively uniqueN-terminal region. Human haspin protein was also demonstrated to have protein kinase activity. The human haspin gene was mapped to chromosome 17p13 by computer database cloning of human genomic DNA, Furthermore, the genomic structure of human hasgin was proven to be intronless and the whole transcription unit was found to be located in an intron of the integrin alpha E2 gene.

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Documento generato il 09/04/20 alle ore 18:36:53