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Titolo:
Steroidogenic acute regulatory protein expression is dependent upon post-translational effects of cAMP-dependent protein kinase A
Autore:
Clark, BJ; Ranganathan, V; Combs, R;
Indirizzi:
Univ Louisville, Sch Med, Dept Biochem & Mol Biol, Louisville, KY 40292 USA Univ Louisville Louisville KY USA 40292 ol Biol, Louisville, KY 40292 USA
Titolo Testata:
MOLECULAR AND CELLULAR ENDOCRINOLOGY
fascicolo: 1-2, volume: 173, anno: 2001,
pagine: 183 - 192
SICI:
0303-7207(20010228)173:1-2<183:SARPEI>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEYDIG TUMOR-CELLS; SIDE-CHAIN CLEAVAGE; ADRENAL-CORTEX CELLS; FACTOR-I; STAR GENE; TRANSCRIPTIONAL REGULATION; PROMOTER ACTIVITY; MITOCHONDRIAL PROTEINS; DEOXYRIBONUCLEIC-ACID; GRANULOSA-CELLS;
Keywords:
StAR; cAMP; PKA; Y1 cells; Kin-8 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Clark, BJ Univ Louisville, Sch Med, Dept Biochem & Mol Biol, Louisville, KY 40292 USA Univ Louisville Louisville KY USA 40292 ouisville, KY 40292 USA
Citazione:
B.J. Clark et al., "Steroidogenic acute regulatory protein expression is dependent upon post-translational effects of cAMP-dependent protein kinase A", MOL C ENDOC, 173(1-2), 2001, pp. 183-192

Abstract

Tropic hormones acutely; stimulate adrenal acid gonadal steroidogenesis byactivation of the cAMP-dependent protein kinase A (PKA) signaling pathway and subsequent induction of Steroidogenic Acute Regulatory (StAR) protein (StAR) expression. We present a comparative study of StAR regulation in mouse adrenocortical Y1 and the derived PKA mutant Kin-X cell lints to evaluatethe PKA requirement for StAR expression. A parallel increase in StAR steady-state mRNA and protein was observed in Y1 cells. StAR mRNA was induced in8-Br-cAMP-treated Kin-8 cells with maximal expression levels approx. 50% of that observed in Y1 cells. However. a corresponding increase in StAR protein, as detected by Western analysis, was absent in the Kin-8 cells. A similar distribution of StAR mRNA in active polysome fractions was observed forboth 8-Br-cAMP-treated Y1 and Kin-8 cells, as well as a 2-fold increase inincorporation of [S-35]methionine into StAR, which indicated translation was not blocked in Kin-8 cells. Together these data indicate that PKA functions at the post-translational level to regulate StAR expression and we propose that phosphorylation of StAR by PKA contributes to protein stability (C) 2001 Elsevier Science Inland Ltd. All rights reserved.

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Documento generato il 23/09/20 alle ore 15:39:51