Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Antecedent protein restriction and high-fat feeding interactively sensitise the leptin response to elevated insulin
Autore:
Holness, MJ; Sugden, MC;
Indirizzi:
Univ London Queen Mary Coll, St Bartholomews & Royal London Sch Med & Dent, Dept Diabet & Metabol Med, London E1 4NS, England Univ London Queen Mary Coll London England E1 4NS London E1 4NS, England
Titolo Testata:
MOLECULAR AND CELLULAR ENDOCRINOLOGY
fascicolo: 1-2, volume: 173, anno: 2001,
pagine: 53 - 62
SICI:
0303-7207(20010228)173:1-2<53:APRAHF>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
OBESE GENE-EXPRESSION; PLASMA LEPTIN; GLUCOSE-METABOLISM; ADIPOSE-TISSUE; SKELETAL-MUSCLE; RAT ADIPOCYTES; FETAL GROWTH; SERUM LEPTIN; HUMANS; SECRETION;
Keywords:
leptin; insulin; protein restriction; high-fat feeding; insulin clamp; glucose utilisation; lipolysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Sugden, MC Univ London Queen Mary Coll, St Bartholomews & Royal London SchMed & Dent, Dept Diabet & Metabol Med, Mile End Rd,Med Sci Bldg, London E14NS, England Univ London Queen Mary Coll Mile End Rd,Med Sci Bldg London England E1 4NS
Citazione:
M.J. Holness e M.C. Sugden, "Antecedent protein restriction and high-fat feeding interactively sensitise the leptin response to elevated insulin", MOL C ENDOC, 173(1-2), 2001, pp. 53-62

Abstract

Using a rat model of moderate: (8 vs. 20% protein) isocaloric protein restriction initiated in early life (low protein, LP). we examined the possiblebasis for the association between impaired early growth and elevated leptin levels in later life in man by examining the acute leptin response to insulin and its relationship with glucose utilisation. We placed subsets of LPrats on a high-saturated-fat (HF) diet containing 20% protein for 4 weeks (LP-4HF) or 8 weeks (LP-8HF), making comparison with age-matched control (C) groups (C, C-4HF. C-8HF). At ambient insulin concentrations, LP was not associated with altered leptinaemia compared with C, despite a more active lipolytic programme as inferred from increased adipocyte sensitivity to norepinephrine. HF feeding led to insulin resistance with respect to whole-bodyglucose disposal (R-d) (measured using [3-H-3] glucose at steady state) inboth LP and C in vivo and impaired suppression of agonist-stimulated lipolysis by insulin in LP but not C in vitro. Whereas insulin infusion for 2 h (while maintaining euglycaemia) only modestly increased plasma leptin levels in vivo in C, C-4HF. C-8HF and LP groups. the leptin response to insulin was greatly enhanced in the HF-fed LP groups. 4 close positive correlation (r = 0.96) existed between plasma leptin levels and R-d in the C groups (viz. C, C4HF. C-8HF) whereas a close inverse correlation (r = 0.95) existed between plasma leptin levels and insulin-stimulated R-d in the LP groups (viz. LP. LP-4HF. LP-8HF). Glucose utilisation (estimated from 2-deoxy-D-[1-H-3] glucose 6-phosphate accumulation) in vivo in two intra-abdominal and twosuperficial adipose-tissue depots was consistently higher in the LP group. After HF feeding, glucose utilisation by the superficial adipose-tissue depots was threefold higher in the LP than in the C group, We conclude that protein restriction from conception to adulthood followed by high-fat feeding sensitises the acute leptin response to insulin, an adaptation associatedwith enhanced glucose utilisation by adipose tissue. This effect is observed despite impaired insulin sensitivity, both at the level of whole-body glucose disposal and adipocyte anti-lipolysis. and increased lipolytic activity (although the latter is not in itself sufficient to influence the leptinresponse). We propose that associations between a low birthweight and elevated leptin concentrations in later life may reflect long-term modulation of adipocyte glucose handling. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 07:29:19