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Titolo:
Biochemical characterization and expression analysis of the Xenopus laeviscorticotropin-releasing hormone binding protein
Autore:
Valverde, RA; Seasholtz, AF; Cortright, DN; Denver, RJ;
Indirizzi:
Univ Michigan, Dept Biol, Ann Arbor, MI 48109 USA Univ Michigan Ann ArborMI USA 48109 , Dept Biol, Ann Arbor, MI 48109 USA Univ Michigan, Dept Biol Chem, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 t Biol Chem, Ann Arbor, MI 48109 USA Univ Michigan, Mental Hlth Res Inst, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 th Res Inst, Ann Arbor, MI 48109 USA
Titolo Testata:
MOLECULAR AND CELLULAR ENDOCRINOLOGY
fascicolo: 1-2, volume: 173, anno: 2001,
pagine: 29 - 40
SICI:
0303-7207(20010228)173:1-2<29:BCAEAO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
PITUITARY-ADRENAL AXIS; HUMAN-PREGNANCY; CRF-BINDING; (CRH)-BINDING PROTEIN; FACTOR RECEPTORS; HUMAN-PLASMA; WEIGHT-GAIN; BRAIN; GENE; METAMORPHOSIS;
Keywords:
CRH; CRH-BP; stress; Xenopus; development;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Denver, RJ Univ Michigan, Dept Biol, 3065C Nat Sci Bldg, Ann Arbor, MI 48109 USA Univ Michigan 3065C Nat Sci Bldg Ann Arbor MI USA 48109 109 USA
Citazione:
R.A. Valverde et al., "Biochemical characterization and expression analysis of the Xenopus laeviscorticotropin-releasing hormone binding protein", MOL C ENDOC, 173(1-2), 2001, pp. 29-40

Abstract

Corticotropin-releasing hormone (CRH) plays a key role in the regulation of responses to stress. The presence of a high affinity binding protein for CRH (CRH-BP) has been reported in mammals. Wr have characterized the biochemical properties and expression of CRH-BP in the South African clawed frog. Xenopus laevis. Appal-ent inhibition constants (K-i[app]) For different ligands were determined by competitive binding assay. Xenopus CRH-BP (xCRH-BP) exhibited a high affinity for xCRH (K-i[app] = 1.08 nM) and sauvagine (1.36 nM). Similar to rodent and human CRH-BPs. the frog protein binds urotensin I and urocortin with high affinity, and ovine CRH with low affinity. RT-PCR analysis showed that xCRH-BP is expressed in brain. pituitary, liver, tail, and intestine. Brain xCRH-BP mRNA is expressed at a relatively constant level throughout metamorphosis and increases slightly in the metamorphic frog. By contrast. the gene is strongly upregulated in the tail at metamorphic climax. Thus, regulation of xCRH-BP gene expression is tissue specific. Because xCRH-BP binds CRH-like peptides with high affinity the protein mayregulated, the bioavailability of CRH in amphibia as it does in mammals. (C) 1001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/20 alle ore 06:56:57