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Titolo:
NXT1 (p15) is a crucial cellular cofactor in TAP-dependent export of intron-containing RNA in mammalian cells
Autore:
Guzik, BW; Levesque, L; Prasad, S; Bor, YC; Black, BE; Paschal, BM; Rekosh, D; Hammarskjold, ML;
Indirizzi:
Univ Virginia, Sch Med, Dept Microbiol, Charlottesville, VA 22908 USA UnivVirginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA Univ Virginia, Myles H Thaler Ctr AIDS & Human Retrovirus Res, Charlottesville, VA 22908 USA Univ Virginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA Univ Virginia, Ctr Cell Signaling, Charlottesville, VA 22908 USA Univ Virginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA Univ Virginia, Dept Biochem & Mol Genet, Charlottesville, VA 22908 USA Univ Virginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA
Titolo Testata:
MOLECULAR AND CELLULAR BIOLOGY
fascicolo: 7, volume: 21, anno: 2001,
pagine: 2545 - 2554
SICI:
0270-7306(200104)21:7<2545:N(IACC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; CONSTITUTIVE TRANSPORT ELEMENT; NUCLEAR-PORE COMPLEX; HIV-1 REV PROTEIN; PLACENTAL ALKALINE-PHOSPHATASE; LATE REPLACEMENT VECTOR; CIS-ACTING ELEMENT; MESSENGER-RNA; BINDING PROTEIN; ENV EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
66
Recensione:
Indirizzi per estratti:
Indirizzo: Hammarskjold, ML Univ Virginia, Sch Med, Dept Microbiol, Box 800734, Charlottesville, VA 22908 USA Univ Virginia Box 800734 Charlottesville VA USA 22908 USA
Citazione:
B.W. Guzik et al., "NXT1 (p15) is a crucial cellular cofactor in TAP-dependent export of intron-containing RNA in mammalian cells", MOL CELL B, 21(7), 2001, pp. 2545-2554

Abstract

TAP, the human homologue of the yeast protein Mex67p, has been proposed toserve a role in mRNA export in mammalian cells. We have examined the ability of TAP to mediate export of Rev response element (RRE)-containing human immunodeficieney virus (HIV) RNA, a well-characterized export substrate in mammalian cells. To do this, the TAP gene was fused in frame to either RevM10 or Rev Delta 78-79. These proteins are nonfunctional Rev mutant proteinsthat can bind to HIV RNA containing the RRE in vivo but are unable to mediate the export of this RNA to the cytoplasm. However, the fusion of TAP to either of these mutant proteins gave rise to chimeric proteins that were able to complement Rev function. Significantly, cotransfection with a vector expressing NXT1 (p15), an NTF2-related cellular factor that binds to TAP, led to dramatic enhancement of the ability of the chimeric proteins to mediate RNA export. Mutant-protein analysis demonstrated that the domain necessary for nuclear export mapped to the C-terminal region of TAP and required the domain that interacts with NXT1, as well as the region that has been shown to interact with nucleoporins. RevM10-TAP function was leptomycin B insensitive. In contrast, the function of this protein was inhibited by Delta CAN, a protein consisting of part of the FG repeat domain of CAN/Nup214. These results show that TAP can complement Rev nuclear export signal function and redirect the export of intron-containing RNA to a CRM1-independent pathway. These experiments support the role of TAP as an RNA export factor in mammalian cells. In addition, they indicate that NXT1 serves as a crucial cellular cofactor in this process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 07:06:17