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Titolo:
Critical role of the HMGI(Y) proteins in adipocytic cell growth and differentiation
Autore:
Melillo, RM; Pierantoni, GM; Scala, S; Batista, S; Fedele, M; Stella, A; De Biasio, MC; Chiappetta, G; Fidanza, V; Condorelli, G; Santoro, M; Croce, CM; Viglietto, G; Fusco, A;
Indirizzi:
Univ Naples, Fac Med & Chirurg, Dipartimento Biol Patol Cellulare & Mol, CNR,Ctr Endocrinol & Oncol Sperimentale, I-80131 Naples, Italy Univ Naples Naples Italy I-80131 col Sperimentale, I-80131 Naples, Italy Ist Nazl Tumori, Fdn Senatore Pascale, I-80131 Naples, Italy Ist Nazl Tumori Naples Italy I-80131 tore Pascale, I-80131 Naples, Italy Univ Studi Catanzaro, Fac Med & Chirurg, Dipartimento Med Sperimentale & Clin, I-88100 Catanzaro, Italy Univ Studi Catanzaro Catanzaro Italy I-88100 n, I-88100 Catanzaro, Italy Thomas Jefferson Univ, Jefferson Med Coll, Kimmel Canc Ctr, Philadelphia, PA 19107 USA Thomas Jefferson Univ Philadelphia PA USA 19107 hiladelphia, PA 19107 USA
Titolo Testata:
MOLECULAR AND CELLULAR BIOLOGY
fascicolo: 7, volume: 21, anno: 2001,
pagine: 2485 - 2495
SICI:
0270-7306(200104)21:7<2485:CROTHP>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENHANCER-BINDING-PROTEIN; NF-KAPPA-B; MOUSE OBESE GENE; IFN-BETA GENE; 3T3-L1 PREADIPOCYTES; TRANSCRIPTION FACTOR; VIRUS INDUCTION; C/EBP FAMILY; DNA-BINDING; HMG I(Y);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Fusco, A Fac Med & Chirurg, Dipartimento Biol & Patol Cellulare & Mol, ViaPansini 5, I-80131 Naples, Italy Fac Med & Chirurg Via Pansini 5 Naples Italy I-80131 ples, Italy
Citazione:
R.M. Melillo et al., "Critical role of the HMGI(Y) proteins in adipocytic cell growth and differentiation", MOL CELL B, 21(7), 2001, pp. 2485-2495

Abstract

The high-mobility group I (HMGI) nonhistone chromosomal proteins HMGI(Y) and HMGI-C have been implicated in defining chromatin structure and in regulating the transcription of several genes. These proteins have been implicated in adipocyte homeostasis: a severe deficiency of fat tissue is found in mice with targeted disruption of the HMGI-C locus, and lipomagenesis in humans is frequently associated with somatic mutations of HMGI genes. The aim of this study was to examine the role of HMGI(Y) proteins in adipocytic cell growth and differentiation. First, we found that differentiation of the preadipocytic 3T3-L1 cell line caused early induction of HMGI(Y) gene expression. Suppression of HMGI(Y) expression by antisense technology dramatically increased the growth rate and impaired adipocytic differentiation in these cells. The process of adipogenic differentiation involves the interplay of several transcription factors, among which is the CCAAT/enhancer-binding protein (C/EBP) family of proteins. These factors are required for the transcriptional activation of adipocyte-specific genes. We also tested the hypothesis that HMGI(Y) might participate in transcriptional control of adipocyte specific promoters. We found that HMGI(Y) proteins bind C/EBP beta in vivo and in vitro. Furthermore, we show that HMGI(Y) strongly potentiates thecapacity of C/EBP beta to transactivate the leptin promoter, an adipose-specific promoter. Taken together, these results indicate that the HMGI(Y) proteins play a critical role in adipocytic cell growth and differentiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/01/20 alle ore 19:57:53