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Titolo:
Strain difference (WKY, SPRD) in the hepatic antioxidant status in rat andeffect of hypertension (SHR, DOCA). Ex vivo and in vitro data
Autore:
Binda, D; Nicod, L; Viollon-Abadie, C; Rodriguez, S; Berthalot, A; Coassolo, P; Richert, L;
Indirizzi:
UFR Med Pharm, Biol Cellulaire Lab, Besancon, France UFR Med Pharm Besancon France rm, Biol Cellulaire Lab, Besancon, France UFR Med Pharm, Physiol Lab, Besancon, France UFR Med Pharm Besancon France Med Pharm, Physiol Lab, Besancon, France F Hoffmann La Roche & Co Ltd, Cardiovasc Dept, CH-4002 Basel, Switzerland F Hoffmann La Roche & Co Ltd Basel Switzerland CH-4002 asel, Switzerland
Titolo Testata:
MOLECULAR AND CELLULAR BIOCHEMISTRY
fascicolo: 1-2, volume: 218, anno: 2001,
pagine: 139 - 146
SICI:
0300-8177(200102)218:1-2<139:SD(SIT>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-PRESSURE; LIPID-PEROXIDATION; NITRIC-OXIDE; GLUTATHIONE-PEROXIDASE; ISOLATED HEPATOCYTES; ENZYME-ACTIVITIES; OXIDATIVE STRESS; IN-VITRO; ACID; ASSAY;
Keywords:
hypertension; liver; antioxidant status; lipid peroxidation; hepatotoxicity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Richert, L Fac Med & Pharm, Biol Cellulaire Lab, Pl St Jacques, F-25030 Besancon, France Fac Med & Pharm Pl St Jacques Besancon France F-25030 , France
Citazione:
D. Binda et al., "Strain difference (WKY, SPRD) in the hepatic antioxidant status in rat andeffect of hypertension (SHR, DOCA). Ex vivo and in vitro data", MOL C BIOCH, 218(1-2), 2001, pp. 139-146

Abstract

We assessed the hepatic antioxidant status of spontaneously (SHR) and desoxicorticosterone acetate (DOCA)-induced hypertensive rats and that of respective normotensive Wistar Kyoto (WKY) and Sprague-Dawley (SPRD) rats. For this we evaluated, ex vivo in liver cytosols, reduced glutathione (GSH) content, glutathione-related enzyme (peroxidase, reductase and transferase) activities as well as the rate of lipid peroxidation in 9-11 week-old rats. The antioxidant status and the cytotoxicity of acetaminophen, a radical- and hydrogen peroxide-mediated hepatotoxic compound, were also assessed in vitro in cultured hepatocytes isolated from hypertensive (SHR, DOCA) and normotensive control (WKY, SPRD) rats. Our results suggest that a difference exists in the hepatic antioxidant status between rat strains, with GSH levels being lower (-15%) and lipid peroxidation rate higher (+30%) in WKY comparedto SPRD rats. In hepatocyte cultures from WKY rats, both GSH content and catalase activity were lower (-30 and -70% respectively) compared to hepatocyte cultures from SPRD rats. This was associated with a 35% higher cytotoxicity of acetaminophen in cultured hepatocytes from WKY rats compared to that in hepatocytes from SPRD rats. Hypertension in DOCA rats (mmHg: 221 +/- 9vs. 138 +/- 5 in control SPRD rats) was associated with decreases (about 30%) in both glutathione peroxidase (GSH-Px) and catalase activities, ex vivo in livers and in vitro in hepatocyte cultures. Hypertension in SHR (mmHg:189 +/- 7 vs. 130 +/- 5 in control WKY rats) was also associated with decreases (about 50%) in GSH-Px activity, ex vivo in livers and in vitro in hepatocyte cultures but catalase activity was not modified. The IC50 of acetaminophen was also lower in hepatocytes from hypertensive rats compared to respective controls, which could be related to the weakened antioxidant status in hepatocytes from hypertensive rats. Our data thus suggest that hepatocyte cultures are appropriated tools in which to assess hepatotoxicity and hepatoprotection in hypertension.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 01:44:19