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Titolo:
Regulation of acp1, encoding a non-aspartyl acid protease expressed duringpathogenesis of Sclerotinia sclerotiorum
Autore:
Poussereau, N; Creton, S; Billon-Grand, G; Rascle, C; Fevre, M;
Indirizzi:
Univ Lyon 1, CNRS, ERS Microbiol & Genet 2009, Lab Biol Cellulaire Fong, F-69622 Villeurbanne, France Univ Lyon 1 Villeurbanne France F-69622 ng, F-69622 Villeurbanne, France
Titolo Testata:
MICROBIOLOGY-UK
, volume: 147, anno: 2001,
parte:, 3
pagine: 717 - 726
SICI:
1350-0872(200103)147:<717:ROAEAN>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARBON CATABOLITE REPRESSION; FUNGAL GENUS VERTICILLIUM; WALL-DEGRADING ENZYMES; EXTRACELLULAR PROTEASES; ASPERGILLUS-NIDULANS; SCYTALIDIUM-LIGNICOLUM; NITROGEN; GENES; PH; SEQUENCE;
Keywords:
acid protease; gene expression; pH regulation; carbon and nitrogen repression; plant pathogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Fevre, M Univ Lyon 1, CNRS, ERS Microbiol & Genet 2009, Lab Biol Cellulaire Fong, Bat 405,43 Bd 11 Novembre 1918, F-69622 Villeurbanne, France Univ Lyon 1 Bat 405,43 Bd 11 Novembre 1918 Villeurbanne France F-69622
Citazione:
N. Poussereau et al., "Regulation of acp1, encoding a non-aspartyl acid protease expressed duringpathogenesis of Sclerotinia sclerotiorum", MICROBIO-UK, 147, 2001, pp. 717-726

Abstract

When grown in the presence of sunflower cell walls, Sclerotinia sclerotiorum, an ubiquitous necrotrophic fungus, secretes several acid proteases including a non-aspartyl protease. The gene acp1, encoding an acid protease, has been cloned and sequenced. The intronless ORF encodes a preproprotein of 252 aa and a mature protein of 200 residues. in vitro expression of acp1 issubject to several transcriptional regulatory mechanisms. Expression induced by plant cell-wall proteins is controlled by both carbon and nitrogen catabolite repression. Glucose on its own represses acp1 expression while ammonium repression requires the simultaneous presence of a carbon source. Ambient ph higher than ph 5 overrides induction resulting in full repression of acp1. These transcriptional regulatory mechanisms and the presence of several motifs in the promoter of acp1 that may encode binding sites for the regulators CREA, AREA and PacC suggest the involvement of these regulators in the control of acp1 expression, acp1 is expressed in planta during sunflower cotyledon infection. Expression is low at the beginning of infection but increases suddenly at the stage of necrosis spreading. Comparison of in vitro and in planta acpl expression suggests that glucose and nitrogen starvation together with acidification can be considered as key factors controlling Sd. sclerotiorum gene expression during pathogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/01/20 alle ore 12:21:08