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Titolo:
Increased susceptibility to apoptosis in circulating lymphocytes of critically ill patients
Autore:
Schroeder, S; Lindemann, C; Decker, D; Klaschik, S; Hering, R; Putensen, C; Hoeft, A; von Ruecker, A; Stuber, F;
Indirizzi:
Univ Bonn, Klin & Poliklin Anasthesiol & Spezielle Intens Me, D-53105 Bonn, Germany Univ Bonn Bonn Germany D-53105 pezielle Intens Me, D-53105 Bonn, Germany Univ Bonn, Inst Klin Biochem, D-53105 Bonn, Germany Univ Bonn Bonn Germany D-53105 Inst Klin Biochem, D-53105 Bonn, Germany Univ Bonn, Chirurg Klin & Poliklin, D-53105 Bonn, Germany Univ Bonn BonnGermany D-53105 rg Klin & Poliklin, D-53105 Bonn, Germany
Titolo Testata:
LANGENBECKS ARCHIVES OF SURGERY
fascicolo: 1, volume: 386, anno: 2001,
pagine: 42 - 46
SICI:
1435-2443(200102)386:1<42:ISTAIC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; TUMOR-NECROSIS-FACTOR; ORGAN FAILURE; ANNEXIN-V; SEPSIS; MICE; LIPOPOLYSACCHARIDE;
Keywords:
apoptosis; peripheral blood lymphocytes; flow cytometry; critically ill patients; severe sepsis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Schroeder, S Univ Bonn, Klin & Poliklin Anasthesiol & Spezielle Intens Me,Sigmund Freud Str 25, D-53105 Bonn, Germany Univ Bonn Sigmund Freud Str 25Bonn Germany D-53105 Germany
Citazione:
S. Schroeder et al., "Increased susceptibility to apoptosis in circulating lymphocytes of critically ill patients", LANG ARCH S, 386(1), 2001, pp. 42-46

Abstract

Background and aims: Lymphocyte apoptosis may influence immune responsiveness in systemic inflammation. Therefore, we investigated whether early signs of apoptosis (i.e., annexin-V binding and cell shrinkage) in peripheral lymphocytes were different among patients with severe sepsis, critically ill, nonseptic patients after major surgery, and healthy individuals. Patients/methods: Ten patients with severe sepsis and ten critically ill, nonsepticpatients after major surgery admitted to a surgical intensive care unit ina university hospital were included in the study. In addition, ten healthyblood donors were included for comparison. We investigated early signs of apoptosis using flow cytometric measurement of annexin-V binding to the cell surface and cell shrinkage of peripheral lymphocytes. Results: The percentage of apoptotic lymphocytes determined as annexin-V positive and propidium iodide negative cells was increased in freshly prepared cells of patientswith severe sepsis (11.4+/-0.5%) and critically ill, nonseptic patients after major surgery (18.5+/-2.0%) relative to healthy blood donors (4.4+/-0.5%) (P<0.05). No significant difference between patients with severe sepsis and patients after major surgery were found. Annexin-V binding increased significantly after OKT-3 stimulation of lymphocytes in patients with severe sepsis (34.4+/-1.6%), patients after major surgery (33.8+/-3.4%), and healthy blood donors (21.1+/-2.8%). No significant difference among groups was detected following OKT-3 stimulation. Furthermore, freshly isolated peripheral lymphocytes of patients with severe sepsis and critically ill, nonsepticpatients after major surgery revealed a significantly higher proportion ofcell shrinkage than in healthy blood donors (55.0+/-2.2%, 21.5+/-2.4% vs 3.6+/-0.7%; P<0.05). Conclusion: Circulating lymphocytes of critically ill patients show a highdegree of el ly signs of cellular apoptosis. This may contribute to hyporesponsiveness of immune cells in systemic inflammation.

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Documento generato il 24/01/20 alle ore 15:46:44